TaqMan real-time polymerase chain reaction for detection of Ophidiomyces ophiodiicola, the fungus associated with snake fungal disease

Table 1 Fungal isolates for which ribosomal RNA regions were analyzed to develop Ophidiomyces ophiodiicola -specific PCR assays

			GenBank Accession No.*
Isolate	Species	ITS Reference	ITS	IGS
CBS 122913	Ophidiomyces ophiodiicola	[8]	EU715819	KP691505
UAMH 6218	Ophidiomyces ophiodiicola	[6]	KF477227	KP691506
UAMH 6642	Ophidiomyces ophiodiicola	[6]	KC884267	KP691507
UAMH 6688	Ophidiomyces ophiodiicola	[6]	KF477228	KP691508
UAMH 9985	Ophidiomyces ophiodiicola	[6]	KF477230	KP691509
UAMH 10296	Ophidiomyces ophiodiicola	[6]	KF477232	KP691510
UAMH 10768	Ophidiomyces ophiodiicola	[6]	KF477234	KP691511
UAMH 10769	Ophidiomyces ophiodiicola	[6]	KF477235	KP691512
UAMH 10949	Ophidiomyces ophiodiicola	[6]	KF477236	KP691513
UAMH 11295	Ophidiomyces ophiodiicola	[6]	KF477237	KP691514
UAMH 10212	Chrysosporium indicum	this study	KP691483	KP691515
CBS 629.79	Chrysosporium sp.	this study	KP691484	KP691516
UAMH 10352	Nannizziopsis guarroi	[6]	KF477208	KP691517
UAMH 10417	Nannizziopsis infrequens	[6,22]	AY744467	KP691518
UAMH 3527	Nannizziopsis vriesii	[6]	KF477198	KP691519
UAMH 10439	Paranannizziopsis australasiensis	[6]	KF477218	KP691520
UAMH 10693	Paranannizziopsis californiensis	[6]	KF477224	KP691521
UAMH 8392	Pseudoamauroascus australiensis	[23]	AJ131787	KP691522

CBS = isolates obtained from the Centraalbureau voor Schimmelcultures.
UAMH = isolates obtained from the University of Alberta Microfungus Collection and Herbarium.
ITS = internal transcribed spacer region of the ribosomal RNA gene complex.
IGS = intergenic spacer region (3′ and 5′ ends) of the ribosomal RNA gene complex.
*All IGS DNA sequences were newly generated for this study.

ISSN: 1746-6148