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Figure 2 | BMC Veterinary Research

Figure 2

From: TaqMan real-time polymerase chain reaction for detection of Ophidiomyces ophiodiicola, the fungus associated with snake fungal disease

Figure 2

Standard curves for Ophidiomyces ophiodiicola real-time PCR assays. Genomic DNA (gDNA) isolated from a pure culture of the type isolate of O. ophiodiicola was quantified, serially diluted, and used as template. All samples were run in triplicate with all points depicting mean Ct values and error bars representing standard deviation. The assays targeting (A) the internal transcribed spacer region (ITS assay) and (B) intergenic spacer region (IGS assay) of O. ophiodiicola were both linear over seven logs, ranging from 5 fg to 5 ng template gDNA (ITS assay: R2 = 0.998; IGS assay: R2 = 0.997).

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