Figure 1From: TaqMan real-time polymerase chain reaction for detection of Ophidiomyces ophiodiicola, the fungus associated with snake fungal diseaseDiagram of fungal ribosomal RNA regions and areas targeted for Ophidiomyces ophiodiicola-specific real-time PCR assays. One assay targets the 3′-end of the internal transcribed spacer region 2 (ITS-2) while the second assay targets the 3′-end of the intergenic spacer region (IGS). Expanded sequences (representing the type isolate of O. ophiodiicola) show the amplicons produced by each PCR and the primer and probe binding sites. Shaded boxes within the sequences represent nucleotide positions that vary between strains of O. ophiodiicola.Back to article page