Table 4 PCR conditions for MIRU-VNTR locus
From: Evaluation of MIRU-VNTR for typing of Mycobacterium bovis isolated from Sika deer in Northeast China
Set and multiplex | PCR conditions |
|---|---|
ALL MIRUs | Start at a denaturing step of 15 min at 95°C, after denaturation, the PCR was performed for 40 cycles of 1 min at 94°C, 1 min at 59°C and 1.5 min at 72°C. Terminated by an incubation of 10 min at 72°C. |
ETRA, C and QUB26 | Start at a denaturing step of 12 min at 95°C, after denaturation, the PCR was performed for 40 cycles of 0.5 min at 94°C, 1 min at 60°C and 2 min at 72°C. Terminated by an incubation of 7 min at 72°C. |
QUB3336, 1895, 2163 | Start at a denaturing step of 15 min at 95°C, after denaturation, the PCR was performed for 40 cycles of 0.5 min at 94°C, 1 min at 55°C and 2 min at 72°C. Terminated by an incubation of 7 min at 72°C. |
Mtub4, 21, 30, 34 | Start at a denaturing step of 15 min at 95°C, after denaturation, the PCR was performed for 40 cycles of 1 min at 94°C, 1 min at 50°C and 0.5 min at 72°C. Terminated by an incubation of 7 min at 72°C. |