Table 1 Sequence of primers used to amplify specific mRNA by real-time RT-PCR
From: Effects of corticosterone on the metabolic activity of cultured chicken chondrocytes
Name | Primer sequence (5′-3′) | Product size (bp) | NCBI Genbank |
|---|---|---|---|
Col X# | Sense: AGTGCTGTCATTGATCTCATGGA | 83 | L11BB9.1 |
Anti-sense: TCAGAGGAATAGAGACCATTGGATT | |||
PTHrP | Sense: CGGAGGATATGATGTTCAC | 79 | AB175678.1 |
Anti-sense: TAGGAGGGCACAGAATAAC | |||
GAPDH | Sense: GAACATCATCCCAGCGTCCA | 132 | NM_204305.1 |
Anti-sense: CGGCAGGTCAGGTCAACAAC |