Figure 3

Profile of cytokines secreted by peripheral blood leukocytes afterin vitrostimulation withLeishmania infantum soluble antigens (SLAg). Short-term whole blood cultures were performed to characterize the cytokine profile secreted by circulating leukocytes from Leishmune® vaccinated dogs at one (T1 = black rectangle), six (T6 = dark grey rectangle) and twelve (T12 = light grey rectangle) months post-vaccination compared to unvaccinated dogs (T0 = white rectangle). Data are reported as cytokine levels in culture supernatants in picograms/mL (pg/mL). Results are expressed as median values over scattering distribution of cytokine levels. Significant differences at p ≤ 0.05 are highlighted by connecting line.