Table 1 Primers used for identifying and completely sequencing the TGEV-HX strain
Primer | Sequence 5′-3′ | Position |
|---|---|---|
TGEV-NF | TCATGCAGATGCCAAATTTAAAGA | 27,213-27,236 |
TGEV-NR | TCATCCTTCTTGTTATTGAATTGT | 27,456-27,479 |
PEDV-NF | TTTCTAAGGTACTTGCAAATAATG | 26,382-26,405 |
PEDV-NR | TTGGAGATCTGGACCTGTTGTTGC | 26,757-26,780 |
P1 | ATGAGTTCCAAACAATTCAAGA | 315-336 |
P2 | ATCAAA ACATCCAAAGCACCCT | 4,318-4,339 |
P3 | AATTCA AAGTCCTAA AAACGAT | 4,250-4,271 |
P4 | GCGTAGATGATCATA AAGAACG | 8,470-8,491 |
P5 | CATTGTCACCCTTGTTGTGAAC | 8,420-8,441 |
P6 | GTAGATGTCAAA AGCTCTACTA | 12,400-12,421 |
P7 | TCTATGCAGAGTTTTACTGTTG | 12,300-12,321 |
P8 | TAATGAATTTATGCTTTGTTCC | 15,200-15,221 |
P9 | AGGCATGTGTGTAGTATGTGGT | 15,100-15,121 |
P10 | AAGCTTAGCAAA AGCTCTT | 18,169-18,187 |
P11 | CGCACTCGCTCTAAATTGTCTT | 18,080-18,101 |
P12 | ACTACGTTTAACCGTTGTCTGT | 20,660-20,681 |
P13 | ATGAAA AAACTATTTGTGG | 20,365-20,383 |
P14 | TTA ATGGACGTGCACTTTT | 24,690-24,708 |
P15 | GCTGTGGATGCATAGGTTGTTT | 24,608-24,629 |
P16 | TTGGAGGGTTATGGGGTTGAAG | 27,021-27,042 |
P17 | CTTCTAAATGGCCAACCAGGGAC | 26,910-26,932 |
P18 | CGAGCATCTCGTTTAGTTCGTT | 28,056-28,077 |
P19 | AGA AAGGTCAGAGCAAGATGTG | 27,963-27,984 |
P20 | GTATCACTATCA AAAGGA AAAT | 28,559-28,580 |
P-F | CAAACTGAATGGAAATAATCAA | 139-160 |
P-R | ATTTGGCAATGCTAGATTTAGTAA | 28,441-28,464 |