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Table 5 Absolute quantification of cytokine and reference gene expression in whole blood samples

From: Design and evaluation of a unique SYBR Green real-time RT-PCR assay for quantification of five major cytokines in cattle, sheep and goats

Target gene Species Cq values Copie number values
Mean Cq SD Cq Mean nc SD nc
IL-4 cattle 28.7 0.3 24 5
goat 28.5 0.2 31 3
sheep 28.1 0.5 39 12
IL-10 cattle 27.2 0.6 156 54
goat 28.2 0.5 48 17
sheep 28.5 0.1 28 1
IL-12B cattle 27.9 0.6 62 21
goat 34.9 1.2 2 0.4
sheep 33.3 0.5 2 0.7
INF-γ cattle 27.4 0.4 106 28
goat 27.7 0.3 103 21
sheep 26.3 0.3 241 52
TNF-α cattle 24.7 0.4 965 199
goat 24.0 0.1 978 34
sheep 24.4 0.1 728 34
GAPDH cattle 18.0 0.3 7.6 104 1.2 104
goat 19.1 0.1 1.6 104 0.1 104
sheep 25.0 0.2 264 38
H3F3A cattle 16.7 0.1 12.5 104 0.7 104
goat 16.3 0.2 14.9 104 2.3 104
sheep 16.2 0.1 19.4 104 1.6 104
ACTB cattle 15.1 0.3 24.5 104 4.3 104
goat 15.4 0.1 32.9 104 2.8 104
sheep 15.5 0.2 30.2 104 4.6 104
PPIA cattle 18.4 0.2 58.0 104 0.6 104
goat 17.7 0.2 75.6 104 0.8 104
sheep 17.7 0.1 65.8 104 0.4 104
YWHAZ cattle 18.8 0.5 70.9 104 1.9 104
goat 23.5 0.3 647 120
sheep 23.6 0.2 482 81
  1. Abbreviations for cytokine and reference genes, see Table 1.
  2. Absolute quantification was checked by amplification of cDNA synthesized from TempusTM total RNA from three different animals of each species. Absolute quantification used the standard curve method and is expressed as the number of copies (nc) and Cq with standard deviation (SD) from six amplification values.