Table 2 Primer characteristics of cytokine and reference genes for bovine, caprine and ovine gene expression
Target gene | Primer sequence (5′-3′) | Optimal primer concentration (nM) | Amplicon size (bp) |
|---|---|---|---|
IL-4 | F-CAGCATGGAGCTGCCT | 300 | 177 |
R-ACAGAACAGGTCTTGCTTGC | 300 | ||
IL-10 | F-CTTTAAGGGTTACCTGGGTTGC | 300 | 239 |
R-CTCACTCATGGCTTTGTAGACAC | 300 | ||
IL-12B | F-CAGCAGAGGCTCCTCTGAC | 600 | 237 |
R-GTCTGGTTTGATGATGTCCCTG | 600 | ||
INF-Îł | F-CAGAGCCAAATTGTCTCCTTC | 300 | 167 |
R-ATCCACCGGAATTTGAATCAG | 300 | ||
TNF-α | F-CCAGAGGGAAGAGCAGTCC | 300 | 111 |
R-GGCTACAACGTGGGCTACC | 300 | ||
GAPDH | F–ATCTCGCTCCTGGAAGATG | 600 | 227 |
R-TCGGAGTGAACGGATTCG | 300 | ||
H3F3A | F-GAGGTCTCTATACCATGGCTC | 300 | 150 |
R-GTACCAGGCCTGTAACGATG | 300 | ||
ACTB | F-TGGGCATGGAATCCTG | 600 | 194 |
R-GGCGCGATGATCTTGAT | 600 | ||
PPIA | F-TGACTTCACACGCCATAAT | 300 | 180 |
R-CTTGCCATCCAACCACTC | 600 | ||
YWHAZ | F-GAAAGGGATTGTGGACCAG | 300 | 183 |
R-GGCTTCATCAAATGCTGTCT | 300 |