Stability of five candidate reference genes in whole blood samples using geNorm and NormFinder analysis. Abbreviations for reference genes, see Table 1. Whole blood (or Tempus™) samples were performed from three different animals of each species. Stability of the five reference genes were calculated in bovine (A), caprine (B) and ovine (C) whole blood samples using geNorm application and NormFinder software. Stability values were calculated as M-value in geNorm and ρ-value in NormFinder. The reference genes were presented in ranked list of the most stable gene to the least stable gene. The pairwise variation (V-score) was performed with geNorm application to determine the optimal number of required reference genes for relative expression in bovine (A), caprine (B) and ovine (C) whole blood samples.