Characterization of a caprine model for the subclinical initial phase of Mycobacterium avium subsp. paratuberculosis infection

Table 4 Lesions in intestinal lymph nodes, oGALT and small intestine of the MAP-inoculated goats at necropsy: macroscopic and histological lesions and MAP detection by IHC

Group	Anim. No.	Mesenteric and ileocolic LNN				oGALT (e.g. JPP, #of sites examined = 7)					Small intestine (#of sites examined = 5)					Lesion cat. summary
Group	Anim. No.	Macro	Histo	MAP IHC	Lesion cat. LNN	Macro	Histo	MAP IHC	#of sites affected	Lesion cat. oGALT	Macro	Histo	MAP IHC	#of sites affected	Lesion cat. intestine	Lesion cat. summary
V1	7	✓, e	G	2	III	-	-	0	0	I	-	-	0	0	I	III
	8	✓, e	G, gInf	2	III	✓	mf , D	2	7	III	✓, E	mf +++	2	4	III	III
	9	✓, e	G, gInf	1	II	✓	mf , C	2	5	III	✓, S	mf ++	0	4	III	III
	10	✓, f	G, gInf	1	III	✓	mf, C	1	4	III	✓, S	mf +	2	4	III	III
	11	✓, e	G, gInf	1	III	✓	f/mf, C/A	0	2	II	-	f +	0	1	II	II
	12	✓, e	G	1	III	-	-	0	0	I	-	-	0	0	I	III
	13	-	gInf	1	II	✓	f/mf, B	0	1	II	✓, MF	mf +	0	2	II	II
V2	14^a	✓, e	G, gInf	1	III	✓	f, C	0	3	II	-	-	0	0	I	II
	15	-	-	0	I	✓	mf, C	0	1	II	✓, MF	-	0	0	I	II
	16^a	✓, e	G, gInf	1	III	✓	mf, B	0	3	II	✓, E	mf ++	0	4	III	III
	17^a	✓, e	G, gInf	1	III	✓	mf, C	0	1	II	-	-	0	0	I	II
	18	✓, e	G, gInf	2	III	✓	mf, C	0	3	II	-	-	0	0	I	II
	19	✓, e	G, gInf	2	III	✓	mf/d, C	0	6	III	✓, S	mf ++	0	4	III	III
	20^a	✓, f	G, gInf	1	II	-	-	0	0	I	-	-	0	0	I	II
V3	27	✓, f	G	0	II	✓	mf, C/A	0	5	III	✓, S, MF	f +	0	1	II	II
	28	✓, e	G, gInf	1	II	✓	mf, C	0	1	II	✓, MF	f ++	0	3	III	II
	29	✓, f	G, gInf	1	II	✓	f, C	0	2	II	-	-	0	0	I	II
	30	✓, f	G, gInf	1	II	✓	mf, C	0	4	III	-	-	0	0	I	II
	31	-	gInf	0	II	-	-	0	0	I	-	-	0	0	I	II
	32	✓, e	G, gInf	1	III	✓	f, C	0	2	II	-	f ++	0	1	II	II
V4	33	✓, e	G, gInf	1	III	✓	f/mf, C/A	0	2	II	✓, MF	mf +	0	1	II	II
	34	-	-	0	I	✓	mf, C	0	1	II	-	f +	0	2	II	II
	35	✓, e	G, gInf	1	III	✓	mf, C	0	7	III	✓, MF	mf ++	0	4	III	III
	36^b	-	-	0	I	✓	mf, C	0	4	III	-	f +	0	1	II	II
	37	✓, e	G, gInf	1	III	✓	mf, D	2	4	III	✓, MF	mf +/++	1	4	III	III
	38	✓, e	G, gInf	1	III	-	-	0	0	I	-	-	0	0	I	III
	39	-	-	0	I	-	-	0	0	I	-	-	0	0	I	I

✓ paratuberculous lesion; − no paratuberculous lesion; LNN lymph nodes; G granuloma; gInf granulomatous infiltrate; MAP IHC MAP detected by IHC; nd not done; ^anecropsy at 37–39 wpi; ^bnecropsy at 35 wpi; 0 no MAP; 1 paucibacillary; 2 multibacillary; f focal; mf multifocal; e extensive; A-D type of histological lesion (see text); +, ++, +++ mild, moderate, severe granulomatous infiltrates; E entire small intestine with thickened intestinal wall and corrugated mucosa; S segments of small intestine with thickened intestinal wall and corrugated mucosa; MF 0.5 – 2 cm thickening of the intestinal mucosa with central depression; lesion categories: I - no lesions; II – mild lesions; III - severe lesions.

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