Characterization of a caprine model for the subclinical initial phase of Mycobacterium avium subsp. paratuberculosis infection

Table 3 Cultural isolation of MAP and lesions in extra-intestinal sites

Group	Animal No.	Liver			Hepatic LN			Spleen			Retropharyngeal LN			Tonsil
Group	Animal No.	Culture	gInf	MAP-IHC	Culture	gInf	MAP-IHC	Culture	gInf	MAP-IHC	Culture	gInf	MAP-IHC	Culture	gInf	MAP-IHC
V1	7	0^a)	0	0	0	0	0	0	0	0	0	0	0	0	0	0
	8	33.33	+++^b)	0	25.00	++	0	12.50	0	0	10.42	0	0	26.04	++	0
	9	0	0	0	13.93	0	0	0	0	0	0	0	0	0	0	0
	10	21.78	++	0	14.16	0	0	11.46	0	0	0	0	0	0	0	0
	11	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
	12	0	+	0	0	0	0	0	0	0	0	0	0	0	0	0
	13	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
V2	14^c)	co.	0	0	0	++	0	0	0	0	0	0	0	0	0	0
	15	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
	16^c)	0	0	0	1.9	+	0	0	0	0	0	0	0	0	0	0
	17^c)	0	0	0	0	++	0	0	0	0	5.26	0	0	0	0	0
	18	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
	19	0	+++	0	6.6	++	0	0	0	0	4.16	+	0	3.57	0	0
	20^c)	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
V3	27	12.50	0	0	8.33	0	0	0	0	0	0	0	0	0	0	0
	28	co.	0	0	0	0	0	0	0	0	0	0	0	0	0	0
	29	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
	30	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
	31	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
	32	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
V4	33	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
	34	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
	35	16.78	0	0	8.33	0	0	0	0	0	8.33	0	0	0	0	0
	36^d)	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
	37	4.29	0	0	22.50	0	0	0	0	0	7.94	0	0	0	0	0
	38	co.	0	0	0	0	0	0	0	0	0	0	0	0	0	0
	39	co.	0	0	0	0	0	0	0	0	0	0	0	0	0	0

^a)Growth Index ^b)granulomatous infiltrate: 0, none; +, mild, focal; ++, moderate, multifocal; +++, severe, diffuse; ^c)necropsy at 37–39 wpi; ^d)necropsy at 35 wpi; co. contaminated; na. not available; gInf, granulomatous infiltrate; MAP IHC, MAP antigen detected by IHC.

ISSN: 1746-6148