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Table 7 Relative fold changes in gene expression in bursae and spleens of SPF chickens infected with vvIBDV strain UPM0081 at 2, 4 and 5 dpi, using a multiplex quantitative GeXP assay

From: Differential modulation of immune response and cytokine profiles in the bursae and spleen of chickens infected with very virulent infectious bursal disease virus

  2 dpi 4 dpi 5dpi
Bursae Spleen Bursae Spleen Bursae Spleen
Pro-inflammatory cytokines and chemokines
IL-1β <2 7.17 ± 2.17b <2 3.26 ± 1.18a <2 9.31 ± 2.59b
IL-6 2.22 ± 0.37a 6.51 ± 0.60b,c 10.96 ± 3.52d 3.35 ± 0.76a,b 9.74 ± 3.48c,d 2.28 ± 0.40a
CCL4 <2 2.61 ± 0.67a,b 4.93 ± 2.05b,c 2.29 ± 0.30a 5.69 ± 2.53c 2.19 ± 0.78a
CXCLi1 <2 2.16 ± 0.39a 4.24 ± 2.24b 2.45 ± 0.62a,b 2.23 ± 0.44a 2.59 ± 0.51a,b
CXCLi2 3.56 ± 1.54a 2.84 ± 0.95a 10.34 ± 3.64b 2.86 ± 1.20a 4.45 ± 1.95a 2.70 ± 1.18a
Th1 cytokines
IFN-γ 9.55 ± 2.29a 12.30 ± 4.75a 26.20 ± 5.23b 9.29 ± 4.24a 26.22 ± 6.78b 9.87 ± 2.92a
IL-12α 4.76 ± 2.69a 23.95 ± 7.96b 10.63 ± 2.58a 15.52 ± 9.23a,b 11.62 ± 3.25a 8.06 ± 4.53a
IL-18 5.69 ± 1.92a 11.16 ± 2.04b 4.03 ± 1.03a 4.38 ± 1.59a 4.90 ± 3.01a 3.97 ± 1.35a
T cell proliferative cytokines
IL-2 <2 <2 -3.57 ± 0.09 <2 <2 <2
IL-15 <2 <2 <2 <2 <2 2.39 ± 0.59
Treg cytokines
IL-10 <2 <2 <2 <2 2.10 ± 0.13 <2
Toll-like receptors
TLR3 <2 <2 -2.33 ± 0.18 <2 <2 <2
TLR7 -2.00 ± 0.11a <2 -3.70 ± 0.09b <2 <2 <2
Others
iNOS 2.32 ± 0.50a 2.53 ± 0.52a 4.27 ± 0.70b,c 2.87 ± 0.91a,b 4.65 ± 1.29c 3.81 ± 0.72a,b,c
MHCI <2 <2 2.14 ± 0.30a 2.24 ± 0.35a <2 <2
MHCII <2 <2 2.93 ± 1.01a 2.08 ± 0.49a <2 <2
TGF-β3 <2 -2.00 ± 0.13a -5.26 ± 0.07b <2 -2.44 ± 0.10a <2
TNFSF13B <2 <2 2.83 ± 0.82a 2.46 ± 0.62a 3.30 ± 1.50a 3.11 ± 0.43a
IL-16 <2 <2 -2.04 ± 0.21a -2.56 ± 0.10a <2 <2
IL-17 F <2 <2 <2 <2 <2 2.00 ± 0.22
  1. Statistical differences between groups were assessed by one-way ANOVA followed by a Duncan post-hoc test. Means labelled with different superscript letters are significantly different (p < 0.05) between organs at different sampling days <2: less than a 2-fold change.