Table 7 Relative fold changes in gene expression in bursae and spleens of SPF chickens infected with vvIBDV strain UPM0081 at 2, 4 and 5 dpi, using a multiplex quantitative GeXP assay

IL-1β

<2

7.17 ± 2.17^b

<2

3.26 ± 1.18^a

<2

9.31 ± 2.59^b

IL-6

2.22 ± 0.37^a

6.51 ± 0.60^b,c

10.96 ± 3.52^d

3.35 ± 0.76^a,b

9.74 ± 3.48^c,d

2.28 ± 0.40^a

CCL4

<2

2.61 ± 0.67^a,b

4.93 ± 2.05^b,c

2.29 ± 0.30^a

5.69 ± 2.53^c

2.19 ± 0.78^a

CXCLi1

<2

2.16 ± 0.39^a

4.24 ± 2.24^b

2.45 ± 0.62^a,b

2.23 ± 0.44^a

2.59 ± 0.51^a,b

CXCLi2

3.56 ± 1.54^a

2.84 ± 0.95^a

10.34 ± 3.64^b

2.86 ± 1.20^a

4.45 ± 1.95^a

2.70 ± 1.18^a

IFN-γ

9.55 ± 2.29^a

12.30 ± 4.75^a

26.20 ± 5.23^b

9.29 ± 4.24^a

26.22 ± 6.78^b

9.87 ± 2.92^a

IL-12α

4.76 ± 2.69^a

23.95 ± 7.96^b

10.63 ± 2.58^a

15.52 ± 9.23^a,b

11.62 ± 3.25^a

8.06 ± 4.53^a

IL-18

5.69 ± 1.92^a

11.16 ± 2.04^b

4.03 ± 1.03^a

4.38 ± 1.59^a

4.90 ± 3.01^a

3.97 ± 1.35^a

IL-2

<2

<2

-3.57 ± 0.09

<2

<2

<2

IL-15

<2

<2

<2

<2

<2

2.39 ± 0.59

IL-10

<2

<2

<2

<2

2.10 ± 0.13

<2

TLR3

<2

<2

-2.33 ± 0.18

<2

<2

<2

TLR7

-2.00 ± 0.11^a

<2

-3.70 ± 0.09^b

<2

<2

<2

iNOS

2.32 ± 0.50^a

2.53 ± 0.52^a

4.27 ± 0.70^b,c

2.87 ± 0.91^a,b

4.65 ± 1.29^c

3.81 ± 0.72^a,b,c

MHCI

<2

<2

2.14 ± 0.30^a

2.24 ± 0.35^a

<2

<2

MHCII

<2

<2

2.93 ± 1.01^a

2.08 ± 0.49^a

<2

<2

TGF-β3

<2

-2.00 ± 0.13^a

-5.26 ± 0.07^b

<2

-2.44 ± 0.10^a

<2

TNFSF13B

<2

<2

2.83 ± 0.82^a

2.46 ± 0.62^a

3.30 ± 1.50^a

3.11 ± 0.43^a

IL-16

<2

<2

-2.04 ± 0.21^a

-2.56 ± 0.10^a

<2

<2

IL-17 F

<2

<2

<2

<2

<2

2.00 ± 0.22