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Table 6 The cell number of IgM + cells, KUL01 + macrophages, CD3 + CD4 + cells and CD3 + CD8 + cells in bursae and spleens of 3-week-old chickens infected with vvIBDV strain UPM0081 at 2, 4 and 5 dpi

From: Differential modulation of immune response and cytokine profiles in the bursae and spleen of chickens infected with very virulent infectious bursal disease virus

  

Bursae (x10 6 cell/bursae)

Spleen (x10 6 cell/spleen)

Average number of cells

Day 0

230.00 ± 2.73a

190.00 ± 3.41a

 

Day 2

173.00 ± 3.64b

150.00 ± 2.76b

 

Day 4

116.00 ± 1.66c

66.40 ± 2.31c

 

Day 5

54.00 ± 1.37d

34.40 ± 2.32d

IgM+

Day 0

189.83 ± 0.83a

57.36 ± 1.21a

 

Day 2

138.99 ± 0.50b

44.35 ± 0.15b

 

Day 4

72.21 ± 0.55c

23.27 ± 1.00c

 

Day 5

35.52 ± 3.07d

14.57 ± 1.55d

KUL-1+

Day 0

9.90 ± 0.52a

6.26 ± 0.13a

 

Day 2

8.76 ± 0.22a

18.54 ± 0.93b

 

Day 4

20.68 ± 2.16b

2.08 ± 0.31c

 

Day 5

12.92 ± 3.50c

2.75 ± 0.49c

CD3+CD4+

Day 0

1.73 ± 0.12a

41.49 ± 0.80a

 

Day 2

1.67 ± 0.09a

33.04 ± 1.51b

 

Day 4

3.82 ± 0.43b

16.52 ± 2.12c

 

Day 5

4.25 ± 0.48b

7.58 ± 0.53d

CD3+CD8+

Day 0

1.67 ± 0.14a

62.36 ± 0.61a

 

Day 2

1.86 ± 0.30a

48.68 ± 0.98b

 

Day 4

3.05 ± 0.38b

20.87 ± 0.66c

 

Day 5

3.52 ± 0.20b

6.81 ± 0.38d

  1. The number of cell (mean ± SD) of the cell subsets were determined by trypan blue cell count and flow cytometry using a FACSCalibur with CellQuest Pro software (BD Bioscience, USA). Statistical differences between groups were assessed by one-way ANOVA followed by a Duncan post-hoc test. Means labelled with different superscript letters are significantly different (p < 0.05) between organs at different sampling days.