BMC Veterinary Research

Table 6 The cell number of IgM ⁺ cells, KUL01 ⁺ macrophages, CD3 ⁺ CD4 ⁺ cells and CD3 ⁺ CD8 ⁺ cells in bursae and spleens of 3-week-old chickens infected with vvIBDV strain UPM0081 at 2, 4 and 5 dpi

From: Differential modulation of immune response and cytokine profiles in the bursae and spleen of chickens infected with very virulent infectious bursal disease virus

		Bursae (x10 ⁶ cell/bursae)	Spleen (x10 ⁶ cell/spleen)
Average number of cells	Day 0	230.00 ± 2.73^a	190.00 ± 3.41^a
	Day 2	173.00 ± 3.64^b	150.00 ± 2.76^b
	Day 4	116.00 ± 1.66^c	66.40 ± 2.31^c
	Day 5	54.00 ± 1.37^d	34.40 ± 2.32^d
IgM⁺	Day 0	189.83 ± 0.83^a	57.36 ± 1.21^a
	Day 2	138.99 ± 0.50^b	44.35 ± 0.15^b
	Day 4	72.21 ± 0.55^c	23.27 ± 1.00^c
	Day 5	35.52 ± 3.07^d	14.57 ± 1.55^d
KUL-1⁺	Day 0	9.90 ± 0.52^a	6.26 ± 0.13^a
	Day 2	8.76 ± 0.22^a	18.54 ± 0.93^b
	Day 4	20.68 ± 2.16^b	2.08 ± 0.31^c
	Day 5	12.92 ± 3.50^c	2.75 ± 0.49^c
CD3⁺CD4⁺	Day 0	1.73 ± 0.12^a	41.49 ± 0.80^a
	Day 2	1.67 ± 0.09^a	33.04 ± 1.51^b
	Day 4	3.82 ± 0.43^b	16.52 ± 2.12^c
	Day 5	4.25 ± 0.48^b	7.58 ± 0.53^d
CD3⁺CD8⁺	Day 0	1.67 ± 0.14^a	62.36 ± 0.61^a
	Day 2	1.86 ± 0.30^a	48.68 ± 0.98^b
	Day 4	3.05 ± 0.38^b	20.87 ± 0.66^c
	Day 5	3.52 ± 0.20^b	6.81 ± 0.38^d

The number of cell (mean ± SD) of the cell subsets were determined by trypan blue cell count and flow cytometry using a FACSCalibur with CellQuest Pro software (BD Bioscience, USA). Statistical differences between groups were assessed by one-way ANOVA followed by a Duncan post-hoc test. Means labelled with different superscript letters are significantly different (p < 0.05) between organs at different sampling days.

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ISSN: 1746-6148

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