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Table 5 Detection of virus RNA in the spleens and bursae of chickens infected with vvIBDV strain UPM0081 by SYBR Green real-time qRT-PCR

From: Differential modulation of immune response and cytokine profiles in the bursae and spleen of chickens infected with very virulent infectious bursal disease virus

Sample

Mean Cq

Viral copy number (log 10)

Spleen

Bursae

Spleen

Bursae

Uninfected

ND*

ND*

ND*

ND*

Day 2

30.42 ± 1.78a

29.13 ± 2.48a,b

10.93a

11.29a,b

Day 4

27.30 ± 0.84b,c

25.92 ± 1.60c

11.81b,c

12.19c

Day 5

21.29 ± 2.13d

19.57 ± 0.21d

13.49d

13.97d

  1. Values are the mean percentages of total cells ± SD per tissue of 5 chickens, each with three technical repeats. Statistical differences between groups were assessed by one-way ANOVA followed by a Duncan post-hoc test. Means labelled with different superscript letters are significantly different (p < 0.05) between organs at different sampling days.
  2. * ND = not detected.