Skip to main content

Table 5 Detection of virus RNA in the spleens and bursae of chickens infected with vvIBDV strain UPM0081 by SYBR Green real-time qRT-PCR

From: Differential modulation of immune response and cytokine profiles in the bursae and spleen of chickens infected with very virulent infectious bursal disease virus

Sample Mean Cq Viral copy number (log 10)
Spleen Bursae Spleen Bursae
Uninfected ND* ND* ND* ND*
Day 2 30.42 ± 1.78a 29.13 ± 2.48a,b 10.93a 11.29a,b
Day 4 27.30 ± 0.84b,c 25.92 ± 1.60c 11.81b,c 12.19c
Day 5 21.29 ± 2.13d 19.57 ± 0.21d 13.49d 13.97d
  1. Values are the mean percentages of total cells ± SD per tissue of 5 chickens, each with three technical repeats. Statistical differences between groups were assessed by one-way ANOVA followed by a Duncan post-hoc test. Means labelled with different superscript letters are significantly different (p < 0.05) between organs at different sampling days.
  2. * ND = not detected.