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Table 4 Relative fold changes in gene expression in HD11 cells infected with different MOI of vvIBDV strain UPM0081 at 6, 24 and 48 hpi

From: Differential modulation of immune response and cytokine profiles in the bursae and spleen of chickens infected with very virulent infectious bursal disease virus

Gene 6 hpi 24 hpi 48 hpi
0.1 MOI 0.5 MOI 0.1 MOI 0.5 MOI 0.1 MOI 0.5 MOI
Pro-inflammatory cytokines and chemokines
IL-1β <2 2.33 ± 0.19a 5.53 ± 0.37b 6.74 ± 1.78b <2 2.92 ± 0.65a
CCL4 2.47 ± 1.03a,b 4.07 ± 0.64a,b 3.89 ± 0.85b 5.07 ± 1.17a 2.69 ± 0.78a 3.29 ± 0.90a
CXCLi1 3.39 ± 0.45a 4.34 ± 0.54b 1.99 ± 0.35c 2.32 ± 0.49c <2 <2
CXCLi2 2.24 ± 0.41a 2.44 ± 0.27a 6.44 ± 0.75b 9.02 ± 0.45c 3.84 ± 0.40d 4.40 ± 1.14d
Th1 cytokines
IL-12α 2.10 ± 0.39a 3.32 ± 0.78b 3.43 ± 0.31b 4.32 ± 1.2b <2 <2
IL-18 <2 3.05 ± 0.53a 2.46 ± 0.23b 3.34 ± 0.50b <2 <2
Treg cytokine
IL-10 -2.44 ± 0.23a -4.17 ± 0.10b -2.00 ± 0.33c -2.86 ± 0.36d <2 -2.22 ± 0.13a,c
Toll-like receptors
TLR2-1 <2 <2 <2 <2 <2 <2
TLR3 <2 2.65 ± 0.37a,b 2.00 ± 0.90a 4.90 ± 0.44c 3.22 ± 0.14b 5.22 ± 0.45c
TLR4 <2 <2 <2 <2 <2 <2
TLR5 <2 <2 <2 <2 <2 <2
TLR7 <2 <2 <2 <2 <2 <2
Others
IL-15 <2 <2 <2 <2 <2 <2
IL-16 <2 <2 <2 -2.13 ± 0.15 <2 <2
iNOS 3.67 ± 0.53a 4.73 ± 0.16b,c 3.52 ± 1.1a,b 7.51 ± 0.73d 5.57 ± 0.61c 5.68 ± 0.44c
MHCI <2 <2 <2 -2.22 ± 0.22a 2.59 ± 1.18b 3.13 ± 1.40b
MHCII -2.33 ± 0.08a -3.13 ± 0.08b <2 -4.55 ± 0.14c <2 -5.26 ± 0.09d
TGF-β3 <2 <2 <2 <2 <2 <2
  1. Statistical differences between groups were assessed by one-way ANOVA followed by a Duncan post-hoc test. Means labelled with different letters are significantly different, p < 0.05. <2: less than a 2-fold change.