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Table 3 Detection of virus RNA in HD11 cells infected with vvIBDV strain UPM0081 by SYBR Green real-time qRT-PCR

From: Differential modulation of immune response and cytokine profiles in the bursae and spleen of chickens infected with very virulent infectious bursal disease virus

Sample Mean Cq Viral copy number (log 10 )
0.1 MOI 0.5 MOI 0.1 MOI 0.5 MOI
Uninfected ND ND ND ND
6 hours 18.89 18.42 14.16 14.29
24 hours 18.85 18.28 14.17 14.33
48 hours 18.05 17.30 14.40 14.61
  1. Statistical differences between groups were assessed by one-way ANOVA followed by a Duncan post-hoc test.
  2. ND = not detected.