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Table 2 Real-time quantitative RT-PCR probes and primers

From: Differential modulation of immune response and cytokine profiles in the bursae and spleen of chickens infected with very virulent infectious bursal disease virus

Target

 

Probe or primer sequence (5′–3′)

Accession no.

Annealing temperature

CXCLi2

Probe

(FAM)-TCTTTACCAGCGTCCTACCTTGCGACA-(BHQ1)

AJ009800

60°C

F

GCCCTCCTCCTGGTTTCAG

 

R

TGGCACCGCAGCTCATT

 

GAPDH

Probe

(FAMa)-CGCCATCACTATCTTCCAGG-(BHQ1)

NM_204305

58°C

Fb

GAACGGGAAACTTGTGAT

 

Rb

GACTCCACAACATACTCA

 

IFN-γ

Probe

(FAM)-TGGCCAAGCTCCCGATGAACGA-(BHQ1)

Y07922

58°C

F

GTGAAGAAGGTGAAAGATATCATGGA

 

R

GCTTTGCGCTGGATTCTCA

 

IL-1β

Probe

(FAM)-CCACACTGCAGCTGGAGGAAGCC-(BHQ1)

AJ245728

60°C

F

GCTCTACATGTCGTGTGTGATGAG

 

R

TGTCGATGTCCCGCATGA

 

IL-10

Probe

(FAM)-CGACGATTCGGCGCTGTCACC-(BHQ1)

AJ621614

58°C

F

CATGCTGCTGGGCCTGAA

 

R

CGTCTCCTTGATCTGCTTGATG

 

IL-12α

Probe

(FAM)-CCAGCGTCCTCTGCTTCTGCACCTT-(BHQ1)

AY262751

58°C

F

TGGCCGCTGCAAACG

 

R

ACCTCTTCAAGGGTGCACTCA

 

IL-18

Probe

(FAM)-CCGCGCCTTCAGCAGGGATG-(BHQ1)

AJ276026

60°C

F

AGGTGAAATCTGGCAGTGGAAT

 

R

ACCTGGACGCTGAATGCAA

 
  1. aFAM, 6-carboxyfluorescein; BHQ1, Black Hole Quencher.
  2. b F, forward primer; R, reverse primer.