|
Gene
|
Accession number
|
Amplicon size (bp)
|
Forward primer sequence* (5′–3′)
|
Reverse primer sequence** (5′–3′)
|
|---|
|
Cytokines and chemokines
|
|
CCL4
|
NM_204720
|
235
|
CTGCTCAAAGCCTGCCATC
|
GTGCAGCCATCCTGAAGC
|
|
CXCLi1
|
NM_205018
|
228
|
CCGATGCCAGTGCATAGAG
|
CCTTGTCCAGAATTGCCTTG
|
|
CXCLi2
|
NM_205498
|
165
|
CCTGGTTTCAGCTGCTCTGT
|
GCGTCAGCTTCACATCTTGA
|
|
GM-CSF
|
NM_001007078
|
242
|
TGAAAACAAATGGGACAGAGG
|
TTCTCCTCTGGGAGCACATC
|
|
IFN-γ
|
NM_205149
|
214
|
GAGCCATCACCAAGAAGATGA
|
TAGGTCCACCGTCAGCTACA
|
|
IL-1β
|
NM_204524
|
137
|
CCAGAAAGTGAGGCTCAACA
|
GTAGCCCTTGATGCCCAGT
|
|
IL-2
|
NM_204153
|
144
|
GTGGCTAACTAATCTGCTGTCCA
|
CCGTAGGGCTTACAGAAAGG
|
|
IL-4
|
NM_001007079
|
151
|
CGTCAAGATGAACGTGACAGA
|
AGGTTCTTGTGGCAGTGCT
|
|
IL-6
|
NM_204628
|
158
|
AGTTCACCGTGTGCGAGAAC
|
TTCGTCAGGCATTTCTCCTC
|
|
IL-10
|
NM_001004414
|
172
|
TAACATCCAACTGCTCAGCTC
|
TGATGACTGGTGCTGGTCTG
|
|
IL-12α
|
NM_213588
|
179
|
AAGGGACTCAACTGCTCCAG
|
TTGTGTTGCTCTGACTGTTGG
|
|
IL-15
|
NM_204571
|
193
|
ATTCCCGATCCAGATTCTGTT
|
ACAGTTGGTACTGGAGACAAATACT
|
|
IL-16
|
NM_204352
|
200
|
GCCTCACAAGAATCAACAACTG
|
TGCTTTGTTCCAACGAGGTC
|
|
IL-17 F
|
NM_204460
|
340
|
TCCATGGGATTACAGGATCG
|
AGGCAAGGCAGTTCTCCTG
|
|
IL-18
|
NM_204608
|
207
|
CGTCAATAGCCAGTTGCTTG
|
CTTCTACCTGGACGCTGAATG
|
|
IL-21
|
NM_001024835
|
186
|
TGTGGTGAAAGATAAGGATGTCG
|
CAGTTTTGGCGAATGTAGCA
|
|
IL-22
|
XM_416079
|
256
|
CAGCCCTACATCAGGAATCG
|
GAACTGTGCCACATCCTCAG
|
|
TGF-β3
|
NM_205454
|
347
|
AATCAGCATACACTGCCCTTG
|
TCGGAAGTCAATGTAAAGAGGAC
|
|
TNFSF13B
|
NM_204327
|
333
|
GGCAAGGTCTCCACTAGAGC
|
TCAGAAGCCAAGGGACAATG
|
|
Toll-like receptors
|
|
TLR2-1
|
NM_204278
|
249
|
TCAGCTACACCAAAATGTTCAACC
|
CGTGATTTTGCCTGTGAGC
|
|
TLR3
|
NM_001011691
|
263
|
TGCATAAGAAGGAGCAGGAAG
|
CTGGCCAGTTCAAGATGCAG
|
|
TLR4
|
NM_001030693
|
270
|
CATCTCTGGAGTTCCTGCTG
|
AGGCTGCTAGACCCAGGTG
|
|
TLR5
|
NM_001024586
|
277
|
CACTCAGGTTCTCGGTATTCG
|
AATCCAGGTGCTTCAGCAAG
|
|
TLR7
|
NM_001011688
|
284
|
GAGTGAGTTATGCCACTCCTCTC
|
TCAAAGGCTTCCACATCAC
|
|
Others
| | | | |
|
iNOS
|
NM_204961
|
221
|
TATGCTCTGCCTGCTGTTGC
|
ATGCAAGTTTGTTGCTTTCC
|
|
MHCI
|
NM_001044683
|
291
|
GGAAACCTGCGTGGAGTG
|
TGGTGACCCAGGTGTGGTA
|
|
MHCII
|
NM_001044679
|
298
|
AGTACGCGCACTTCGACA
|
AGAAGCCCGTCACGTAGC
|
|
GAPDH
|
NM_204305
|
312
|
CTGGCAAAGTCCAAGTGGTG
|
AGCACCACCCTTCAGATGAG
|
|
KAN
r***
|
-
|
325
|
ATCATCAGCATTGCATTCGATTCCTGTTTG
|
ATTCCGACTCGTCCAACATC
|
- *Forward universal primer sequence (AGGTGACACTATAGAATA).
- **Reverse universal primer sequence (GTACGACTCACTATAGGGA).
- ***Internal control.
