Skip to main content

Table 1 GeXP primers sequence and amplicon sizes designed for quantification of chicken cytokines, chemokines and other immune-related genes

From: Differential modulation of immune response and cytokine profiles in the bursae and spleen of chickens infected with very virulent infectious bursal disease virus

Gene

Accession number

Amplicon size (bp)

Forward primer sequence* (5′–3′)

Reverse primer sequence** (5′–3′)

Cytokines and chemokines

CCL4

NM_204720

235

CTGCTCAAAGCCTGCCATC

GTGCAGCCATCCTGAAGC

CXCLi1

NM_205018

228

CCGATGCCAGTGCATAGAG

CCTTGTCCAGAATTGCCTTG

CXCLi2

NM_205498

165

CCTGGTTTCAGCTGCTCTGT

GCGTCAGCTTCACATCTTGA

GM-CSF

NM_001007078

242

TGAAAACAAATGGGACAGAGG

TTCTCCTCTGGGAGCACATC

IFN-γ

NM_205149

214

GAGCCATCACCAAGAAGATGA

TAGGTCCACCGTCAGCTACA

IL-1β

NM_204524

137

CCAGAAAGTGAGGCTCAACA

GTAGCCCTTGATGCCCAGT

IL-2

NM_204153

144

GTGGCTAACTAATCTGCTGTCCA

CCGTAGGGCTTACAGAAAGG

IL-4

NM_001007079

151

CGTCAAGATGAACGTGACAGA

AGGTTCTTGTGGCAGTGCT

IL-6

NM_204628

158

AGTTCACCGTGTGCGAGAAC

TTCGTCAGGCATTTCTCCTC

IL-10

NM_001004414

172

TAACATCCAACTGCTCAGCTC

TGATGACTGGTGCTGGTCTG

IL-12α

NM_213588

179

AAGGGACTCAACTGCTCCAG

TTGTGTTGCTCTGACTGTTGG

IL-15

NM_204571

193

ATTCCCGATCCAGATTCTGTT

ACAGTTGGTACTGGAGACAAATACT

IL-16

NM_204352

200

GCCTCACAAGAATCAACAACTG

TGCTTTGTTCCAACGAGGTC

IL-17 F

NM_204460

340

TCCATGGGATTACAGGATCG

AGGCAAGGCAGTTCTCCTG

IL-18

NM_204608

207

CGTCAATAGCCAGTTGCTTG

CTTCTACCTGGACGCTGAATG

IL-21

NM_001024835

186

TGTGGTGAAAGATAAGGATGTCG

CAGTTTTGGCGAATGTAGCA

IL-22

XM_416079

256

CAGCCCTACATCAGGAATCG

GAACTGTGCCACATCCTCAG

TGF-β3

NM_205454

347

AATCAGCATACACTGCCCTTG

TCGGAAGTCAATGTAAAGAGGAC

TNFSF13B

NM_204327

333

GGCAAGGTCTCCACTAGAGC

TCAGAAGCCAAGGGACAATG

Toll-like receptors

TLR2-1

NM_204278

249

TCAGCTACACCAAAATGTTCAACC

CGTGATTTTGCCTGTGAGC

TLR3

NM_001011691

263

TGCATAAGAAGGAGCAGGAAG

CTGGCCAGTTCAAGATGCAG

TLR4

NM_001030693

270

CATCTCTGGAGTTCCTGCTG

AGGCTGCTAGACCCAGGTG

TLR5

NM_001024586

277

CACTCAGGTTCTCGGTATTCG

AATCCAGGTGCTTCAGCAAG

TLR7

NM_001011688

284

GAGTGAGTTATGCCACTCCTCTC

TCAAAGGCTTCCACATCAC

Others

    

iNOS

NM_204961

221

TATGCTCTGCCTGCTGTTGC

ATGCAAGTTTGTTGCTTTCC

MHCI

NM_001044683

291

GGAAACCTGCGTGGAGTG

TGGTGACCCAGGTGTGGTA

MHCII

NM_001044679

298

AGTACGCGCACTTCGACA

AGAAGCCCGTCACGTAGC

GAPDH

NM_204305

312

CTGGCAAAGTCCAAGTGGTG

AGCACCACCCTTCAGATGAG

KAN r***

-

325

ATCATCAGCATTGCATTCGATTCCTGTTTG

ATTCCGACTCGTCCAACATC

  1. *Forward universal primer sequence (AGGTGACACTATAGAATA).
  2. **Reverse universal primer sequence (GTACGACTCACTATAGGGA).
  3. ***Internal control.