Feeding a high-grain diet reduces the percentage of LPS clearance and enhances immune gene expression in goat liver

Table 2 The list of primers for amplification of RT-qPCR

Gene	Forward primer	Reverse primer	Length
TLR4	CTGAGAACCGAGAGCTGGGAC	GCCTTGAAATGTGTTGTCTTCA	207 bp
TLR2	GCTCAGGTGGAAGCTTTCCAG	GGTGATCTCGTTGTTGGACAG	241 bp
IL-1A	GATGATGACCTGGAAGCCATTG	GCTGAGAATCCTCTTCTGATAC	259 bp
IL-1B	CCGTGATGATGACCTGAGGAG	CAAGACAGGTATAGATTCTTGTC	303 bp
IL-6	CGAAGCTCTCATTAAGCACATC	CCAGGTATATCTGATACTCCAG	241 bp
TNF-α	CAACAGGCCTCTGGTTCAGAC	GGACCTGCGAGTAGATGAGG	209 bp
IL-8	CTGAGAGTTATTGAGAGTGGGC	CAGTACTCAAGGCACTGAAGTAG	259 bp
IL-10	GTGATGCCACAGGCTGAGAAC	GAAGATGTCAAACTCACTCATGG	213 bp
CCL5	CTACACCAGCAGCAAGTGCT	CAAGCTGCTTAGGACAAGAGG	190 bp
CCL20	GAAGCAGCAAGCAGCTTTGAC	GTTCCATTCCAGGGAGCATC	244 bp
SAA3	GACATTCCTCAGGGAAGCTG	CTTCGAATCCTTCCGTACCTG	247 bp
Hp	GGAGTACTCGGTTCGCTATCA	CCATCGTTCATTGATGAGTGTG	280 bp
LBP	GAGCTGTCCACCACCAAGATG	CACACTCAGATCAAATGTACCG	243 bp

ISSN: 1746-6148