Table 2 Sequences of primers used in the PCR assays
Primer | Sequence 5’-3’ | Reference |
|---|---|---|
PRRSV ORF7 gene | [26] | |
PRRSV-F | CCAAATAACAACGGCAAGCA | |
PRRSV-R | ATGCTGAGGGTGATGCTGTGA | |
PCV2 ORF2 gene | [27] | |
PCV2.S4 | CACGGATATTGTAGTCCTGGT | |
PCV2.A4 | CCGCACCTTCGGATATACTGTC | |
HEV ORF2 gene | [3] | |
HEV- externer primer | AATTATGCYCAGTAYCGRGTTG | |
HEV- externer primer | CCCTTRTCYTGCTGMGCATTCTC | |
HEV- interner primer | GTWATGCTYTGCATWCATGGCT | |
HEV- interner primer | AGCCGACGAAATCAATTCTGTC | |
CSFV E2 gene | [28] | |
E2- externer primer | GCATCAACCAYKGCATTCC | |
E2- externer primer | GTCTGTGTGGGTRATTAAGTTCCCTA | |
E2- interner primer | CTRGTRACTGGGGCACAAGG | |
E2- interner primer | ACCAGCRGCGAGTTGYTCTG | |
PEDV S gene | [29] | |
P1 | TTCTGAGTCACGAACAGCCA | |
P2 | CATATGCAGCCTGCTCTGAA | |
TGEV S gene | [29] | |
T1 | GTGGTTTTGGTYRTAAATGC | |
T2 | CACTAACCAACGTGGARCTA | |
PRV gB genes | [30] | |
PRVF | GGGGTTGGACAGGAAGGACACCA | |
PRVR | AACCAGCTGCACGCGCTCAA | |
PPV NS1 gene | [30] | |
PPVF | AGTTAGAATAGGATGCGAGGAA | |
PPVR | AGAGTCTGTTGGTGTATTTATTGG |