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Table 1 Characteristics of gene specific primers used for qPCR

From: Treatment of lactating sows with clofibrate as a synthetic agonist of PPARα does not influence milk fat content and gains of litters

Gene

Forward primer (from 5′ to 3′)

PCR product size (bp)

NCBI GenBank

Slope

R 2

Efficiency

M-value

Reverse primer (from 5′ to 3′)

Reference genes

  ACTB

GACATCCGCAAGGACCTCTA

205

XM_003124280.3

−3.76

1.000

0.84

0.034

 

ACATCTGCTGGAAGGTGGAC

      

  ATP5G1

CAGTCACCTTGAGCCGGGCGA

94

NM_001025218.2

−3.43

0.999

0.99

0.033

 

TAGCGCCCCGGTGGTTTGC

      

  GSR

AGCGCGATGCCTACGTGAGC

175

XM_003483635.2

−3.43

0.997

0.96

0.038

 

GGTACGCCGCCTGTGGCAAT

      

  RPS9

GTCGCAAGACTTATGTGACC

325

XM_005664825.1

−3.78

0.999

0.84

0.034

 

AGCTTGAAGACCTGGGTCTG

      

PPARα target genes

  ACOX1

CTCGCAGACCCAGATGAAAT

218

NM_001101028.1

−3.53

0.999

0.92

 
 

TCCAAGCCTCGAAGATGAGT

      

  CD36

TCCTCTGACATTTGCAGGTCAATCT

107

NM_001044622.1

−3.59

0.999

0.90

 
 

GGAGATGCAAAAGCTGTGGATGG

      

  CPT1A

GCATTTGTCCCATCTTTCGT

198

NM_001129805.1

−3.46

0.999

0.95

 
 

GCACTGGTCCTTCTGGGATA

      

  CPT1B

CACACTGCAGCACCTCACA

183

NM_001007191.1

−4.09

1.000

0.76

 
 

GTGAGGGCTGCCAGCTTTT

      

  CYP4A24

GGTTTGCTCCTGTTGAATGG

121

NM_214424.1

−3.34

1.000

0.99

 
 

GCATCACTTGGACAGACTTG

      

  FABP1

ATCGTGCAGAATGGGAAGCA

133

NM_001004046.1

−3.43

0.999

0.96

 
 

ACTGAACCACTGTCTTGACC

      

  FABP3

CAACATGACCAAGCCTACCA

227

NM_001099931.1

−3.36

1.000

0.98

 
 

CTAGTTCCCGAACAAGCGTT

      

  LPL

TTCTCCCGACGACGCAGATTTT

166

NM_214286.1

−3.36

0.988

0.98

 
 

TGCAATCACACGGATGGCTTCT

      

  SLC27A1

GGTTCCAGCCTGTTGAATGT

275

NM_001083931.1

−3.38

0.992

0.98

 
 

AACAAAACCTTGGTGCTTGG

      

  UCP3

GCCACTTTGTCTCTGCCTTC

219

NM_214049.1

−3.39

1.000

0.97

 
 

CAAACATCACCACGTTCCAG

      

Genes of the ubiquitin proteasome system

  FBXO32

TCACAGCTCACATCCCTGAG

167

NM_001044588.1

−3.30

0.994

1.01

 
 

GACTTGCCGACTCTCTGGAC

      

  TRIM63

ATGGAGAACCTGGAGAAGCA

219

NM_001184756.1

−3.62

0.998

0.89

 
 

ACGGTCCATGATCACCTCAT

      

  UBB

GGTGGCTGCTAATTCTCCAG

127

NM_001105309.1

−3.51

1.000

0.93

 
 

TTTTGGACAGGTTCAGCTATTAC

      
  1. Abbreviations: ACOX1 acyl-CoA oxidase, ACTB  actin, beta, ATP5G1 ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C1, CD36 fatty acid translocase, CPT1 carnitine-palmitoyl-transferase, CYP4A24 cytochrome P450 A24, FABP fatty acid binding protein, FBXO32 F-box protein 32, LPL lipoprotein lipase, GSR glutathione reductase, PPARα peroxisome proliferator- activated receptor α, RPS9 ribosomal protein S9, SLC27A1 solute carrier family 27 (fatty acid transporter), member 1, TRIM63 tripartite motif containing 63, E3 ubiquitin protein ligase, UBB ubiquitin B, UCP3 uncoupling protein 3.
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BMC Veterinary Research

ISSN: 1746-6148

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