Immunofluorescence of HeLa cell cultures inoculated with FBS medium-free (top) and fecal extracts from diseased animals (below). The HeLa cells were fixed the day 4 after infection. Immunofluorescence staining was done using anti-Cav-1 MoAb (first column). Transmission microscopy images were used to see the location and structure of the HeLa cells (second column). The merged images shown in the last column were generated with Photoshop S5 software. Pictures were taken at 600x magnification and scale bar represents 10 um.