Figure 2From: Rapid and specific detection of porcine parvovirus using real-time PCR and High Resolution Melting (HRM) analysis Real time PCR and HRM analysis of serial diluted genomic DNA of porcine parvovirus (PPV). (a) Melting peaks of amplicon from 12 positive field samples, 80.77 ± 0.04°C. (b) Melting peaks of amplicons of 10 fold diluted genomic DNA was 80.76 ± 0.06°C between 100 ng and 1 pg, while it is 81.28°C when the diluted DNA is 0.1 pg.Back to article page