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Figure 1 | BMC Veterinary Research

Figure 1

From: Rapid and specific detection of porcine parvovirus using real-time PCR and High Resolution Melting (HRM) analysis

Figure 1

Ten fold diluted genomic DNA of porcine parvovirus (PPV) tested with two kind of real time PCR (100 ng to 0.1 pg). (a) TaqMan real time PCR amplification for 10 fold diluted genomic DNA; (b) Real time PCR with HRM analysis for 10 fold diluted genomic DNA; (c) A linear regression of the data providing a formula of y = -3.1929x + 40.387 (R2 = 0.9972) between Cp value and log concentration (100 ng to 1 pg), when performed with TaqMan real time PCR; (d) A linear regression of the data providing a formula of y = -3.1332x + 38.875 (R2 = 0.9974), between Cp value and log concentration (100 ng to 1 pg) when performed with real time PCR coupled with HRM analysis.

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