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Table 3 Primers used in MiIFN-α and mature peptide (mMiIFN-α) gene PCR assays

From: Cloning, expression and antiviral activity of mink alpha-interferons

Gene fragment Predicted size (bp) Primers Tm( ° C)
MiIFN-α1–13 564 P1 5′-ATGGCCCTGCCCTGCTCCT- 3′ 50.1
P2 5′-TCACTTCCTGCTCCGCAATC-3′
mMiIFN-α1–13 495 P3 5′- CGGGATCCTGTGACCTGCCTCAG-3′ 55
P4 5′- CCAAGCTTTCACTTCCTGCTCCGCAAT-3′