Table 3 Primers used in MiIFN-α and mature peptide (mMiIFN-α) gene PCR assays
From: Cloning, expression and antiviral activity of mink alpha-interferons
Gene fragment | Predicted size (bp) | Primers | Tm( ° C) |
|---|---|---|---|
MiIFN-α1–13 | 564 | P1 5′-ATGGCCCTGCCCTGCTCCT- 3′ | 50.1 |
P2 5′-TCACTTCCTGCTCCGCAATC-3′ | |||
mMiIFN-α1–13 | 495 | P3 5′- CGGGATCCTGTGACCTGCCTCAG-3′ | 55 |
P4 5′- CCAAGCTTTCACTTCCTGCTCCGCAAT-3′ |