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Table 2 Serology and real-time PCR results based on stage of bovine besnoitiosis a

From: Natural Besnoitia besnoiti infections in cattle: chronology of disease progression

Animal data       
Animal ID   SA 4 SA 6 SA 8 SA 20 SA 22
Breed   S S S L L
Age (months)   20 19 13 53 49
Pregnancy status   np np np p p
Study entry (td)   1 1 1 3 51
Acute disease       
Start/End (dps b)   −9/2 −6/6 subclinical unknown/4 unknown/0
Ct (+/− 3 dps)c Lowest/highest/mean (# sample) 32.5/36.7/33.9 (4) 30.4/36.0/33.8 (7) 39.1/ND/ND (1) 29.5/31.4/30.3 (5) 31.5/ND/ND (1)
IFAT (+/− 3 dps) Lowest/highest/mean (# sample) 100/400/260 (5) 50/400/229 (7) 50/800/ND (2) 800/1600/1280 (5) 400/400/ND (2)
Subacute disease       
Start/End (dps)   3/18 7/31 subclinical 5/22 1/11
Ct (7–18 dps)c Lowest/highest/mean (# sample) 34.5/45.0/ND (2) 33.6/35.3/ND (2) 45.0/45.0/45.0 (3) 28.0/32.9/ND (2) 31.6/ND/ND (1)
IFAT (7–18 dps)d Lowest/highest/mean (# sample) 800/1600/1120 (5) 400/1600/867 (6) 800/800/800 (3) 1600/1600/ND (2) 1600/ND/ND (1)
Chronic disease       
Start (dps)   19 32 subclinical 23 12
Ct (32–152 dps)c Lowest/highest/mean (# sample) 45.0 /45.0/45.0 (4) 23.2/37.6/27.6 (5) 27.4/45.0/41.5 (9) 15.5/18.8/16.9 (4) 11.6/19.5/15.3 (3)
IFAT (32–152 dps) Lowest/highest/mean (# sample) 1600/3200/2200 (8) 800/3200/1829 (7) 800/1600/1244 (9) 1600/6400/3556 (9) 6400/6400/6400 (5)
  1. aAbbreviations: SA = study animal; S = Simmental; L = Limousin; np = not pregnant; p = pregnant; td = trial day; dps = days post seroconversion; ND = not determined; PCR = polymerase chain reaction; IFAT = immunofluorescent antibody test; Ct = Cycle threshold in BbRT2 PCR [30].
  2. bData on the date of seroconversion are previously published [29].
  3. cFor determination of mean ct-values, negative real-time PCR results were depicted at cycle threshold [Ct] = 45.0.
  4. dFor SA 22 only laboratory results between 7 to 11 dps were regarded.