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Table 2 Serology and real-time PCR results based on stage of bovine besnoitiosis a

From: Natural Besnoitia besnoiti infections in cattle: chronology of disease progression

Animal data

      

Animal ID

 

SA 4

SA 6

SA 8

SA 20

SA 22

Breed

 

S

S

S

L

L

Age (months)

 

20

19

13

53

49

Pregnancy status

 

np

np

np

p

p

Study entry (td)

 

1

1

1

3

51

Acute disease

      

Start/End (dps b)

 

−9/2

−6/6

subclinical

unknown/4

unknown/0

Ct (+/− 3 dps)c

Lowest/highest/mean (# sample)

32.5/36.7/33.9 (4)

30.4/36.0/33.8 (7)

39.1/ND/ND (1)

29.5/31.4/30.3 (5)

31.5/ND/ND (1)

IFAT (+/− 3 dps)

Lowest/highest/mean (# sample)

100/400/260 (5)

50/400/229 (7)

50/800/ND (2)

800/1600/1280 (5)

400/400/ND (2)

Subacute disease

      

Start/End (dps)

 

3/18

7/31

subclinical

5/22

1/11

Ct (7–18 dps)c

Lowest/highest/mean (# sample)

34.5/45.0/ND (2)

33.6/35.3/ND (2)

45.0/45.0/45.0 (3)

28.0/32.9/ND (2)

31.6/ND/ND (1)

IFAT (7–18 dps)d

Lowest/highest/mean (# sample)

800/1600/1120 (5)

400/1600/867 (6)

800/800/800 (3)

1600/1600/ND (2)

1600/ND/ND (1)

Chronic disease

      

Start (dps)

 

19

32

subclinical

23

12

Ct (32–152 dps)c

Lowest/highest/mean (# sample)

45.0 /45.0/45.0 (4)

23.2/37.6/27.6 (5)

27.4/45.0/41.5 (9)

15.5/18.8/16.9 (4)

11.6/19.5/15.3 (3)

IFAT (32–152 dps)

Lowest/highest/mean (# sample)

1600/3200/2200 (8)

800/3200/1829 (7)

800/1600/1244 (9)

1600/6400/3556 (9)

6400/6400/6400 (5)

  1. aAbbreviations: SA = study animal; S = Simmental; L = Limousin; np = not pregnant; p = pregnant; td = trial day; dps = days post seroconversion; ND = not determined; PCR = polymerase chain reaction; IFAT = immunofluorescent antibody test; Ct = Cycle threshold in BbRT2 PCR [30].
  2. bData on the date of seroconversion are previously published [29].
  3. cFor determination of mean ct-values, negative real-time PCR results were depicted at cycle threshold [Ct] = 45.0.
  4. dFor SA 22 only laboratory results between 7 to 11 dps were regarded.