deletion associated with epidermolysis bullosa in Hereford cattle. a) Screenshot of the next generation sequence reads mapped against the reference sequencing and visualized with Integrative Genome Viewer. Note the 2433 bp deletion including the first exon of LAMC2. b) We used a common forward primer in combination with a reverse primer specific for the deleted region (Rev1) and a reverse primer located immediately downstream of the deletion (Rev2) for PCR based deletion genotyping. Detected genotypes of the LAMC2 deletion. c) The diagnostic PCR performed on genomic DNA allows genotype differentiation. The gel picture shows the affected calf (del/del), three heterozygous parents (wt/del), and a normal control (wt/wt).