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Table 2 Primers used for amplification of FMDV VP1 cDNA in this study

From: Characterisation of recent foot-and-mouth disease viruses from African buffalo (Syncerus caffer) and cattle in Kenya is consistent with independent virus populations

Primer name

Sequence (5ˈ-3ˈ)

Isolate of origin

Reference

1.0 PN 15 (NK-72) (reverse)

GAAGGGCCCAGGGTTGGACTC

Accession no. AJ 539141

Mason et al., 2003 [35].

13-KPN 100 (forward)

GGGTGGBBGTSTWMCAGRTSACMGACAC

K127/2011

This study

13-KPN 101(forward)

CACTGCTAYCAYKCNGARTGGGA

K10/2012

This study

1.0-U PN E (AKS-2) (forward)

TTAACTACCACTTCATGTACACXG

Accession no AY593849

Sangula et al., 2010 [36].

  1. V = A,C,G; H = A,C,T; B = C,G,T; S = C,G; W = A,T; M = A,C; R = A,G; Y = C,T; K = G,T; N = Any; X = Inosine.
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