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Table 3 Primers used in PCR to detect the virulence genes in Salmonella strains, according to Skyberg et al. [9]

From: Prevalence and genetic characteristics of Salmonella in free-living birds in Poland

Genes Function of gene Sequence of nucleotides Size
spvB Growth within host F-CTATCAGCCCCGCACGGAGAGCAGTTTTTA 717 bp
R-GGAGGAGGCGGTGGCGGTGGCATCATA
spiA Survival within macrophage F-CCAGGGGTCGTTAGTGTATTGCGTGAGATG 550 bp
R-CGCGTAACAAAGAACCCGTAGTGATGGATT
pagC Survival within macrophage F-CGCCTTTTCCGTGGGGTATGC 454 bp
R-GAAGCCGTTTATTTTTGTAGAGGAGATGTT
cdtB Host recognition/invasion F-ACAACTGTCGCATCTCGCCCCGTCATT 268 bp
R-CAATTTGCGTGGGTTCTGTAGGTGCGAGT
msgA Survival within macrophage F-GCCAGGCGCACGCGAAATCATCC 189 bp
R-GCGACCAGCCACATATCAGCCTCTTCAAAC
invA Host recognition/invasion F-CTGGCGGTGGGTTTTGTTGTCTTCTCTATT 1070 bp
R-AGTTTCTCCCCCTCTTCATGCGTTACCC
sipB Entry into nonphagocytic cells F-GGACGCCGCCCGGGAAAAACTCTC 875 bp
R-ACACTCCCGTCGCCGCCTTCACAA
prgH Host recognition/invasion F-GCCCGAGCAGCCTGAGAAGTTAGAAA 756 bp
R-TGAAATGAGCGCCCCTTGAGCCAGTC
span Entry into nonphagocytic cells F-AAAAGCCGTGGAATCCGTTAGTGAAGT 504 bp
R-CAGCGCTGGGGATTACCGTTTTG
orgA Host recognition/invasion F-TTTTTGGCAATGCATCAGGGAACA 255 bp
R-GGCGAAAGCGGGGACGGTATT
tolC Host recognition/invasion F-TACCCAGGCGCAAAAAGAGGCTATC 161 bp
R-CCGCGTTATCCAGGTTGTTGC
iron Iron acquisition F-ACTGGCACGGCTCGCTGTCGCTCTAT 1205 bp
R-CGCTTTACCGCCGTTCTGCCACTGC
sitC Iron acquisition F-CAGTATATGCTCAACGCGATGTGGGTCTCC 768 bp
R-CGGGGCGAAAATAAAGGCTGTGATGAAC
lpfC Host recognition/invasion F-GCCCCGCCTGAAGCCTGTGTTGC 641 bp
R-AGGTCGCCGCTGTTTGAGGTTGGATA
sifA Filamentous structure formation F-TTTGCCGAACGCGCCCCCACACG 449 bp
R-GTTGCCTTTTCTTGCGCTTTCCACCCATCT
sopB Host recognition/invasion F-CGGACCGGCCAGCAACAAAACAAGAAGAAG 220 bp
R-TAGTGATGCCCGTTATGCGTGAGTGTATT
pefA Host recognition/invasion F-GCGCCGCTCAGCCGAACCAG 157 bp
R-GCAGCAGAAGCCCAGGAAACAGTG