Table 3 Primers used in PCR to detect the virulence genes in Salmonella strains, according to Skyberg et al. [9]
From: Prevalence and genetic characteristics of Salmonella in free-living birds in Poland
Genes | Function of gene | Sequence of nucleotides | Size |
|---|---|---|---|
spvB | Growth within host | F-CTATCAGCCCCGCACGGAGAGCAGTTTTTA | 717 bp |
R-GGAGGAGGCGGTGGCGGTGGCATCATA | |||
spiA | Survival within macrophage | F-CCAGGGGTCGTTAGTGTATTGCGTGAGATG | 550 bp |
R-CGCGTAACAAAGAACCCGTAGTGATGGATT | |||
pagC | Survival within macrophage | F-CGCCTTTTCCGTGGGGTATGC | 454 bp |
R-GAAGCCGTTTATTTTTGTAGAGGAGATGTT | |||
cdtB | Host recognition/invasion | F-ACAACTGTCGCATCTCGCCCCGTCATT | 268 bp |
R-CAATTTGCGTGGGTTCTGTAGGTGCGAGT | |||
msgA | Survival within macrophage | F-GCCAGGCGCACGCGAAATCATCC | 189 bp |
R-GCGACCAGCCACATATCAGCCTCTTCAAAC | |||
invA | Host recognition/invasion | F-CTGGCGGTGGGTTTTGTTGTCTTCTCTATT | 1070 bp |
R-AGTTTCTCCCCCTCTTCATGCGTTACCC | |||
sipB | Entry into nonphagocytic cells | F-GGACGCCGCCCGGGAAAAACTCTC | 875 bp |
R-ACACTCCCGTCGCCGCCTTCACAA | |||
prgH | Host recognition/invasion | F-GCCCGAGCAGCCTGAGAAGTTAGAAA | 756 bp |
R-TGAAATGAGCGCCCCTTGAGCCAGTC | |||
span | Entry into nonphagocytic cells | F-AAAAGCCGTGGAATCCGTTAGTGAAGT | 504 bp |
R-CAGCGCTGGGGATTACCGTTTTG | |||
orgA | Host recognition/invasion | F-TTTTTGGCAATGCATCAGGGAACA | 255 bp |
R-GGCGAAAGCGGGGACGGTATT | |||
tolC | Host recognition/invasion | F-TACCCAGGCGCAAAAAGAGGCTATC | 161 bp |
R-CCGCGTTATCCAGGTTGTTGC | |||
iron | Iron acquisition | F-ACTGGCACGGCTCGCTGTCGCTCTAT | 1205 bp |
R-CGCTTTACCGCCGTTCTGCCACTGC | |||
sitC | Iron acquisition | F-CAGTATATGCTCAACGCGATGTGGGTCTCC | 768 bp |
R-CGGGGCGAAAATAAAGGCTGTGATGAAC | |||
lpfC | Host recognition/invasion | F-GCCCCGCCTGAAGCCTGTGTTGC | 641 bp |
R-AGGTCGCCGCTGTTTGAGGTTGGATA | |||
sifA | Filamentous structure formation | F-TTTGCCGAACGCGCCCCCACACG | 449 bp |
R-GTTGCCTTTTCTTGCGCTTTCCACCCATCT | |||
sopB | Host recognition/invasion | F-CGGACCGGCCAGCAACAAAACAAGAAGAAG | 220 bp |
R-TAGTGATGCCCGTTATGCGTGAGTGTATT | |||
pefA | Host recognition/invasion | F-GCGCCGCTCAGCCGAACCAG | 157 bp |
R-GCAGCAGAAGCCCAGGAAACAGTG |