Zhangfei induces differentiation of canine osteosarcoma cells. D–17 and Abrams cells were either mock-infected or infected with Adeno-ZF or Adeno-LacZ. The positive control cells were treated with 10-5 mM vitamin D3. The mRNA levels of osteoblast differentiation marker (osteocalcin) were estimated by qRT-PCR. Standard deviations from means and P values as calculated using a Student T-test are shown.