Figure 2From: The effect of Zhangfei/CREBZF on cell growth, differentiation, apoptosis, migration, and the unfolded protein response in several canine osteosarcoma cell lines Ectopic expression of Zhangfei suppresses cell growth in canine osteosarcomas. D–17, Abrams, McKinley, and Gracie canine OS cells were mock-infected or infected with adenovirus vectors expressing either Zhangfei (Adeno-ZF) or β-galactosidase (Adeno-LacZ) and growth rates were measured (A) by absorbance at 405 nm with WST-1 at different time points after infection. Error bars indicate standard deviations from means of three individual experiments. Standard deviations from means of three individual experiments are shown and significance (*P < 0.05, **P < 0.01) was determined using ANOVA. (B). Zhangfei was detected by immunoblotting using antiserum against Zhangfei.Back to article page