Ectopic expression of Zhangfei suppresses cell growth in canine osteosarcomas. D–17, Abrams, McKinley, and Gracie canine OS cells were mock-infected or infected with adenovirus vectors expressing either Zhangfei (Adeno-ZF) or β-galactosidase (Adeno-LacZ) and growth rates were measured (A) by absorbance at 405 nm with WST-1 at different time points after infection. Error bars indicate standard deviations from means of three individual experiments. Standard deviations from means of three individual experiments are shown and significance (*P < 0.05, **P < 0.01) was determined using ANOVA. (B). Zhangfei was detected by immunoblotting using antiserum against Zhangfei.