Effects of ribavirin on the replication and genetic stability of porcine reproductive and respiratory syndrome virus

Table 1 Sequences of primers used for PCR amplification and sequencing of the nsp2 and ORF5 regions in the VR2332 genome

Sequenced region	Primer name	Nucleotide location ^a	Sequences (5′-3′)	Sequenced length (bp)
nsp2	_pnsp2F	1249-1268	CCTCCTCAGAATAAGGGTTG	3588
	_pnsp2R	5120-5138	TGTCAAGGGCAGGGTAAG
	1a 1481R	1463-1481	GGGAGTAGTGTTTGAGGTG
	1a 1366F	1366-1383	CTCTTGTGCGACTGCTAC
	1a 2115R	2097-2115	TACAGGTCAATCTTTGCTG
	1a 2058F	2058-2075	CCCAGAACAAAACCAACC
	1a 2867R	2850-2867	ATTGCGGTGAGGACACAA
	1a 2771F	2771-2788	TGGGAAGATTTGGCTGTT
	1a 3581R	3563-3581	CAATGGTAAGGTCGCTCTC
	1a 3511F	3511-3529	TCCGTGTGAGTTTGTGATG
	1a 4276R	4258-4276	CAGTAACCTGCCAAGAATG
	1a 4141F	4141-4158	CGCTGCTTGTGAGTTTGA
ORF5	_pP5F^r	13716-13734	CCTGAGACCATGAGGTGGG	603
ORF5	_pP5R^r	14457-14479	TTTAGGGCATATATCATCACTGG	603

a: Location of primers in the full-length VR2332 genome (GenBank accession [AY150564]). p: primers used for PCR amplification and sequencing. The remaining primers were used only for sequencing. r: reference primers used in a previous study [17].

ISSN: 1746-6148