Figure 1From: Effects of ribavirin on the replication and genetic stability of porcine reproductive and respiratory syndrome virus Evaluation of the effects of four antiviral mutagens on PRRSV replication. The effects of different concentrations of ribavirin (A), 5-fluorouracil (C), 5-azacytidine (E), and amiloride (G) in RPMI-1640 medium on the replication of the PRRSV isolate VR2332 in MARC-145 cells were evaluated, as determined by the production of progeny viruses (TCID50/ml) over time. Cytotoxicity assays were performed using cell culture fluids collected from MARC-145 cells every 12 hours after being incubated with the following mutagens: ribavirin (B), 5-fluorouracil (D), 5-azacytidine (F), and amiloride (H), as indicated in the panel. The results are expressed as the luminescence (RLU) from dead-cell protease activity. Asterisks represent a significant difference (p < 0.05) in virus replication after mutagen treatment compared to that after vehicle treatment.Back to article page