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Table 3 Mean, SD and percentiles of laboratory parameters of B . besnoiti seronegative and B . besnoiti seropositive Limousin cattle

From: Natural Besnoitia besnoiti infections in cattle: hematological alterations and changes in serum chemistry and enzyme activities

  Seronegative Seropositive
Hematology Mean ± SD Percentiles Mean ± SD Percentiles
2.5th 97.5th 2.5th 97.5th
RBCs (T/l) 8.44 ± 1.9a 6.13 13.70 7.08 ± 1.2 a,b 5.08 9.76
Hemoglobin (mmol/l) 7.5 ± 0.7 6.1 8.9 7.9 ± 1.3a 5.8 10.7
HCT (%) 35.5 ± 3.5 30.0 41.3 36.3 ± 5.6a 23.0 45.4
MCV (fl) 43.9 ± 9.4 25.9 58.1 52.2 ± 3.9 b 43.9 58.8
MCH (fmol) 0.9 ± 0.2 0.5 1.3 1.1 ± 0.1 b 0.9 1.3
MCHC (mmol/l) 21.2 ± 0.9 19.8 23.2 21.5 ± 1.4a 19.2 24.6
WBCs (G/l) 7.34 ± 1.6 4.90 11.00 7.31 ± 2.94a 2.83 14.44
Serum chemistry
Total protein (g/l) 68.4 ± 5.9 60.1 81.7 76.0 ± 6.9 b 63.1 89.1
Albumin (g/l) 32.9 ± 4.4 26.0 41.2 36.4 ± 2.7 b 31.5 41.3
Globulin (g/l) 35.3 ± 4.3 28.7 43.8 40.3 ± 7.0 a,b 28.5 55.5
A/G ratio 0.95 ± 0.18a 0.73 1.39 0.94 ± 0.19a 0.63 1.38
Urea (mmol/l) 3.9 ± 1.3a 2.6 7.0 5.2 ± 1.9 a,b 2.2 9.6
Creatinine (μmol/l) 163.6 ± 21.2 124.7 207.7 164.4 ± 27.1a 125.4 214.6
Total bilirubin (μmol/l) 2.6 ± 0.9 1.0 4.4 2.9 ± 1.2a 0.9 5.5
Sodium (mmol/l) 145.8 ± 3.4 139.0 153.0 141.1 ± 3.1 b 135.5 146.5
Potassium (mmol/l) 5.3 ± 0.9 3.6 6.7 4.8 ± 0.7 b 3.4 6.0
Calcium (mmol/l) 2.3 ± 0.1 2.1 2.6 2.3 ± 0.2 1.9 2.6
Phosphorus (mmol/l) 1.6 ± 0.3 1.1 2.3 1.9 ± 0.4 b 1.3 2.8
Magnesium (mmol/l) 0.6 ± 0.2 0.3 1.1 0.9 ± 0.1 a,b 0.7 1.1
Chloride (mmol/l) 99.2 ± 2.3 95.6 103.2 96.7 ± 2.6 b 91.6 101.5
Enzymes
AST (IU/l) 155.1 ± 61.9a 68.9 323.4 102.2 ± 31.6 a,b 58.2 166.0
GGT (IU/l) 16.6 ± 6.6 6.8 28.7 19.5 ± 9.1 1.7 35.4
GLDH (IU/l) 14.7 ± 7.1a 5.2 29.6 12.7 ± 8.1a 4.0 33.2
CK (IU/l) 702.8 ± 466.8a 78.0 2092.0 331.9 ± 283.1 a,b 106.5 885.0
  1. Samples from B. besnoiti seronegative Limousins comprise 41 samples from 41 animals, samples from B. besnoiti seropositive Limousins comprise 75 samples (hematology) respectively 65 samples (serum chemistry/enzymes) from 54 animals. SD = standard deviation, a = normality test not passed, b = significantly (P < 0.01) different versus seronegative Limousins (Mann–Whitney-Test) highlighted in bold.