Figure 1

Optimization of annealing temperature (a), primer concentration (b), and plasmid DNA concentration (c) for MEV nanoPCR. Lane M: Low DNA Mass Ladder (Invitrogen, Carlsbad, USA); (a) lanes 1–12: The annealing temperatures were 48°C, 48.6°C, 49.4°C, 50.6°C, 52.2°C, 53.7°C, 54.9°C, 56.3°C, 57.8°C, 58.8°C, 59.5°C, and 60°C, respectively. (b) lanes 1–10: The primer volumes were 0.1 μL, 0.2 μL, 0.3 μL, 0.4 μL, 0.5 μL, 0.6 μL, 0.7 μL, 0.8 μL, 0.9 μL, and 1.0 μL, respectively. (c) lanes 1–10: The plasmid DNA volumes were 0.1 μL, 0.2 μL, 0.4 μL, 0.6 μL, 0.8 μL, 1.0 μL, 1.2 μL, 1.4 μL, 1.6 μL, and 1.8 μL, respectively.