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Table 1 Primers and PCR conditions

From: Enhanced Wnt/β-catenin and Notch signalling in the activated canine hepatic progenitor cell niche

Gene

Direction

Sequence (5’- 3’)

Tm (°C)

Product size (bp)

Genbank accession number

B2MGa

Forward

TCCTCATCCTCCTCGCT

60.3

85

XM_535458

 

Reverse

TTCTCTGCTGGGTGTCG

   

HPRTa

Forward

AGCTTGCTGGTGAAAAGGAC

58

114

NM_001003357

 

Reverse

TTATAGTCAAGGGCATATCC

   

RPS5a

Forward

TCACTGGTGAGAACCCCCT

62.5

141

XM_533568

 

Reverse

CCTGATTCACACGGCGTAG

   

RPS19a

Forward

CCTTCCTCAAAAAGTCTGGG

61

95

XM_533657

 

Reverse

GTTCTCATCGTAGGGAGCAAG

   

FZD1

Forward

GGCGCAGGGCACCAAGAAG

58.8

97

XM_539411

 

Reverse

GAGCGACAGAATCACCCACCAGA

   

TCF3

Forward

GGTGAATGAGCGGGTCCTGAACA

58.8

128

XM_849145

 

Reverse

TGAGCTGGCTGGCACGGTAGTC

   

AXIN2

Forward

CACCCGCTCTACAACAAGGT

60

128

XM_548025

 

Reverse

AGGTGGAGATGAAGCACAGC

   

NOTCH1

Forward

TACCGGCCAGAACTGTGAGGAGAA

56

108

XM_537795

 

Reverse

GGAGGGCAGCGGCAGTTGTAAGTA

   

NOTCH2

Forward

AGCACGCATCCTGGCATACCTC

58.3

106

XM_853135

 

Reverse

TGGGGATTAGCTGGAAAGTCACAA

   

NOTCH3

Forward

TCTGCCAGAGTTCCGTGGTG

66.8

117

XM_847948

 

Reverse

ATGGGGTACAAGGGCTGCTG

   

NOTCH4

Forward

GGAAGGGAGCCAGGGACCAACACA

68

96

NM_004557

 

Reverse

TCAGGGCCACAGCGGGACAAATC

   

JAG1

Forward

GGGCAACACCTTCAATCTCAAG

58.5

122

XM_853730

 

Reverse

CATTACTGGAATCCCACGCTTC

   

JAG2

Forward

GGGTACGTGCGTGGGC

64

 

XM_548004

 

Reverse

CACCGTTGTAGCAAGGCAG

   

HEY1

Forward

CCAGGAAAAGACGAAGAGGC

62.5

226

NM_001002953

 

Reverse

CTCCGATAGTCCATAGCAAGGG

   
  1. Table legend text. aReference genes.