Figure 4
From: Treatment with embryonic stem-like cells into osteochondral defects in sheep femoral condyles
Femur, sheep. Embryonic Stem-like cells engraftment at 24 months from surgery. A) Histological aspect: Azan-Mallory staining; 2× magnification; bar: 500 μm. B) Fluorescent in situ hybridization (FISH): positive signals in chondrocytes derived from ES-like cells. 40× magnification; bar: 50 μm. C) Same field, 60× magnification; bar: 10 μm. D) Normal female adult articular cartilage from the right lateral femoral condyle (negative control). No signals are detected within chondrocytes. 20× magnification; bar: 30 μm. E) Same field, 40× magnification; bar: 20 μm. F) Dot-Blot test with SRY probe. K-: spot female sheep fibroblasts (negative control), K+: spot male sheep fibroblasts (positive control), 1–2: spot female ES-like cells, 3: spot male ES-like cells.