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Figure 1 | BMC Veterinary Research

Figure 1

From: Treatment with embryonic stem-like cells into osteochondral defects in sheep femoral condyles

Figure 1

Embryo sex determination and ES-like colonies gene expression . A): Embryo sex determination. Lanes 1, 8, 9, 16: DNA marker 100–1000 bp ladder (Sigma); lane 2: negative control (no DNA); lane 3: female positive control (sheep oviductal cells); lane 4: male positive control (male lamb fibroblasts); lanes 5, 10, 12, 13: female embryos; lanes 6, 7, 11, 14, 15: male embryos. B,C, D, E) ES-like colonies gene expression. B) Oct4gene expression; C) Sox2 gene expression; D) Nanog gene expression; E) Stat3 gene expression. Lane 1: DNA Marker 100–1000 bp ladder (Sigma); lane 2: negative control (no cDNA); lane 3: in vitro produced sheep blastocyst, used as positive control; lane 4: ES-like cells.

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