Table 1 Presence of parasite DNA in different small intestinal tissues, lymph nodes and blood of sheep orally infected with 3000 T. gondii PRU tachyzoites and euthanized at different days post infection (dpi)
Time | Parasite load as determined by RT qPCR and expressed as log 10 of parasite DNA/10 6cells | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
DPI | 4 | 8 | 10 | 14 | 21 | |||||||
Animals | Sh1 | Sh2 | Sh3 | Sh4 | Sh5 | Sh6 | Sh7 | Sh8 | Sh9 | Sh10 | Sh11 | |
Duodenal E | 0,36 | nd | nd | 3,06 | -2,26 | -3,06 | -2,23 | -2,90 | nd | nd | nd | |
Duodenal LP | -0,43 | nd | nd | 2,88 | -1,60 | -1,46 | -2,59 | 2,74 | nd | nd | -1,58 | |
Duodenal PP | nd | 0,11 | nd | 2,26 | -1,15 | -2,68 | -0,74 | 4,09 | nd | nd | nd | |
Jejunal E | nd | nd | nd | 2,23 | -0,64 | nd | -2,67 | -1,54 | nd | nd | -0,67 | |
Jejunal LP | nd | nd | nd | nd | -0,02 | -1,67 | nd | -1,96 | nd | nd | nd | |
Jejunal PP | nd | nd | nd | nd | -1,23 | nd | -2,07 | 3,40 | nd | nd | nd | |
Ileal E | nd | -0,16 | nd | 0,87 | -1,44 | nd | -3,29 | -1,86 | nd | nd | nd | |
Ileal LP | nd | nd | nd | nd | -0,44 | -1,91 | -4,00 | -2,10 | nd | nd | nd | |
Ileal PP | nd | nd | nd | nd | -2,00 | nd | nd | 2,95 | nd | nd | nd | |
Duodenal MLN | 0,34 | nd | nd | 1,95 | -0,31 | -1,20 | -0,77 | 2,76 | 0,05 | nd | nd | |
Jejunal MLN | 2,63 | nd | nd | 1,45 | -0,51 | -0,99 | -0,94 | 3,67 | 8,37 | nd | nd | |
Ileal MLN | nd | nd | nd | 2,44 | -0,72 | -2,87 | -1,06 | 3,19 | -1,29 | nd | nd | |
Peripheral blood MC | 0,45 | nd | nd | nd | nd | nd | -1,68 | -1,30 | nd | nd | nd | |
Popliteal LN | nd | nd | nd | nd | -1,54 | -1,66 | -0,89 | nd | nd | nd | nd | |
Splenocytes | nd | nd | nd | nd | -2,49 | nd | -2,00 | nd | nd | nd | nd | |