Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalisin dairy cattle in Ningxia, northwestern China

Table 1 Numbers of fecal samples examined with microscopy for Cryptosporidium oocysts and Giardia cysts on Chinese farms

Farm	Sample size	Prevalence (%) Cryptosporidium / Giardia	Cryptosporidium species (no.)	Giardia duodenalis assemblage (no.)
1	45	2.22%/2.22%	C. bovis (1)*	B2 (1)*
2	32	9.38%/6.25%	C. parvum (1)*, C. bovis* (2)**	B2 (2)*
3	57	3.51%/3.51%	C. parvum (2)***	E (1), B1 (1)***
4	46	4.35%/6.52%	C. parvum (2)	E (3)
5	63	3.17%/7.94%	C. parvum (2)	E (5)
6	106	0.94%/1.89%	C. parvum (1)	E (2)
7	110	6.36%/3.64%	C. parvum (7)	E (4)
8	166	0/1.20%		E (2)
9	94	4.26%/3.19%	C. andersoni (4)	E (3)
10	32	0/6.25%		E (2)
11	141	0/1.42%		E (2)
12	76	0/1.32%		E (1)
13	69	1.45%/0	C. bovis (1)
14-20	329	0/0
Total	1366	1.68%/2.12%	C. parvum (15), C. bovis (4), C. andersoni (4)

The distributions of Cryptosporidium spp. and G. duodenalis assemblages were determined with PCR–RFLP analysis of the SSU rRNA gene and sequence analysis of the tpi and gdh genes.
*Sample positive for a mixed infection of C. parvum and G. duodenalis subtype B2.
**One sample showed a mixed infection of C. parvum and G. duodenalis subtype B2, and another sample showed a mixed infection of C. bovis and G. duodenalis subtype B2.
***Sample positive for a mixed infection of C. parvum and G. duodenalis subtype B1.

ISSN: 1746-6148