Histological and immunohistochemical characterization of the inflammatory and glial cells in the central nervous system of goat fetuses and adult male goats naturally infected with Neospora caninum

Table 2 Neosporosis in goats, lesions, diagnosis and immunolabelling in adult male goats

Goat	Age (months)	Lesions			Parasite confirmation			Immunolabelling
		Gliosis	P. cuffs	M.G.C	PCR	HE	IHQ	RCA	GFAP	PCNA	MHC-II	CD3	CD79α
1	12	+++	+++	+	+	-	-	+++	+++	+	+++	+	-
2	6	+	+	-	+	+	+	-	++	-	-	+	-
3	12	+++	+++	+	+	+	+	+++	+	++	++	++	+
4	12	+	++	+	+	+	+	++	-	-	++	-	-
5	6	-	+	-	+	-	-	-	-	-	-	+	-
6	36	-	+	-	+	-	-	-	-	-	+	+	-
7*	10	-	-	-	-	-	-	-	-	-	-	-	-

Lesions graduation: discrete (+), moderate (++) and severe (+++). Immunohistochemistry labelling graduated by the number of cells in a field of 400x: less than 10 cells per field (+), 10 to 30 cells per field (+ +), more than 30 cells per field (+ + +). *Negative Control. P. cuffs (perivascular cuffs), M.G.C (Multinucleated giant cells), HE (Hematoxilin and Eosin staining), PCR (Polimerase chain reaction), IHQ (Immunohistochemistry).

ISSN: 1746-6148