Neosporosis in goats: central nervous system lesions in naturally infected animals. A. Lectin histochemical staining (RCA1) revealed predominant microglia in a focus of gliosis; the same area is shown in Figure 1A (fetus 4) (streptavidin–peroxidase method); bar = 100 μm. B. Immunohistochemical labeling of N. caninum, showing a cyst in the central nervous system (goat 3) (biotin–streptavidin–peroxidase method); bar = 50 μm. C. Immunolabeling of GFAP in the glial focus shown in Figure 1B (goat 1) (dual link system–horseradish peroxidase [HRP] method); bar = 100 μm. D. Cerebral cortex: MHC-II immunolabeling in the perivascular cuff and adjacent gliosis (goat 1) (dual link system–HRP method); bar = 100 μm.