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Table 2 Detection of CSFV in tonsil extracts

From: Differential detection of classical swine fever virus challenge strains in C-strain vaccinated pigs

(A) CBR/93 (B) UK2000/7.1
Pig group and number Blood viraemia Tonsil RNA levels (log 10copies/ng) Clinical outcome End day (dpi) Pig group and number Blood viraemia Tonsil RNA levels (log 10copies/ng) Clinical outcome End day (dpi)
CBR/93 C-strain Total CSFV UK2000/7.1 C-strain Total CSFV
Mock vaccination* Mock vaccination*
A.1 High 7.10 0 8.62 Euthanized 17 E.1 High 9.39 0 9.43 Euthanized 17
A.2 Moderate 4.99 0 8.21 Euthanized 21 E.2 Moderate 8.50 0 8.87 Euthanized 15
A.3 High 6.23 0 8.18 Euthanized 21 E.3 High 8.35 0 8.56 Euthanized 15
A.4 Moderate 6.02 0 8.06 Euthanized 17 E.4 High 7.98 0 8.48 Euthanized 17
Vaccination -5 dpi Vaccination -5 dpi
B.1 Low < assay 4.61 5.19 Healthy 25 F.1 Low 0 3.64 4.38 Healthy 29
B.2 0 2.76 3.62 Healthy 25 F.2 0 3.23 3.61 Healthy 29
B.3 0 1.61 3.76 Healthy 30 F.3 0 2.93 3.42 Healthy 30
B.4 0 1.92 3.25 Healthy 30 F.4 0 2.19 3.41 Healthy 30
B.5 0 1.66 2.96 Healthy 30 F.5 0 1.81 0.87 Healthy 29
B.6 0 2.21 2.87 Healthy 25 F.6 Low 0 1.83 0.11 Healthy 30
Vaccination -3 dpi Vaccination -3 dpi*
C.1 Low < assay 0 4.17 Healthy 33 G.1 High 7.79 2.75 8.33 unrelated inf. 22
C.2 Low 0 1.65 3.74 Healthy 32 G.2 Low 8.01 0 8.23 Healthy 28
C.3 0 1.24 3.45 Healthy 33 G.3 Low 2.75 4.29 5.13 Healthy 28
C.4 Low 0.5 1.45 3.43 Healthy 32 G.4 1.06 3.80 4.78 Healthy 24
C.5 Low < assay 1.35 3.16 Healthy 32 G.5 Low 2.63 1.74 4.08 Healthy 24
C.6 0 0 2.51 Healthy 33 G.6 1.98 1.12 3.35 Healthy 24
Vaccination -1 dpi* Vaccination -1 dpi*
D.1 High 7.28 0 8.69 Euthanized 19 H.1 High 7.51 0 8.69 Euthanized 19
D.2 Moderate 7.75 4.26 8.68 Euthanized 12 H.2 High 7.98 0 8.21 Euthanized 12
D.3 Moderate 3.03 < assay 5.62 Healthy 23 H.3 Moderate 7.99 4.16 8.16 Euthanized 11
D.4 Low 2.28 1.02 5.10 unrelated inf. 19 H.4 Low 4.65 0 5.43 Healthy 23
D.5 Low 0.76 2.53 4.15 Healthy 23 H.5 Low 2.59 1.00 4.41 Healthy 23
D.6 Low < assay < assay 2.77 Healthy 23 H.6 Low 2.67 1.58 4.16 Healthy 23
  1. *Naïve animals co-housed together with these groups became CSFV infected.
  2. Pigs were mock-vaccinated or vaccinated at 1, 3, or 5 days before inoculation with (A) the CBR/93 or (B) the UK2000/7.1 field strains. Blood viraemia is recorded as not detectable (), low, moderate or high. The amount of CBR/93, UK20007.1 or C-strain vaccine RNA determined using differential real-time qRT-PCR or of total CSFV RNA quantified using a routine real-time qRT-PCR assay is shown as log10 viral RNA copy per ng 18S RNA. Bold type indicates high copy number. Samples with high Ct values below the quantifiable limit of an assay (< assay) or no Ct value (0) are indicated. The clinical outcome shows those animals that reached the humane endpoint and were euthanized. Animals indicated as healthy had clinical scores that did not exceed 4. Tonsil samples were obtained on the indicated days post inoculation (dpi).