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Table 2 Detection of CSFV in tonsil extracts

From: Differential detection of classical swine fever virus challenge strains in C-strain vaccinated pigs

(A) CBR/93

(B) UK2000/7.1

Pig group and number

Blood viraemia

Tonsil RNA levels (log 10copies/ng)

Clinical outcome

End day (dpi)

Pig group and number

Blood viraemia

Tonsil RNA levels (log 10copies/ng)

Clinical outcome

End day (dpi)

CBR/93

C-strain

Total CSFV

UK2000/7.1

C-strain

Total CSFV

Mock vaccination*

Mock vaccination*

A.1

High

7.10

0

8.62

Euthanized

17

E.1

High

9.39

0

9.43

Euthanized

17

A.2

Moderate

4.99

0

8.21

Euthanized

21

E.2

Moderate

8.50

0

8.87

Euthanized

15

A.3

High

6.23

0

8.18

Euthanized

21

E.3

High

8.35

0

8.56

Euthanized

15

A.4

Moderate

6.02

0

8.06

Euthanized

17

E.4

High

7.98

0

8.48

Euthanized

17

Vaccination -5 dpi

Vaccination -5 dpi

B.1

Low

< assay

4.61

5.19

Healthy

25

F.1

Low

0

3.64

4.38

Healthy

29

B.2

0

2.76

3.62

Healthy

25

F.2

0

3.23

3.61

Healthy

29

B.3

0

1.61

3.76

Healthy

30

F.3

0

2.93

3.42

Healthy

30

B.4

0

1.92

3.25

Healthy

30

F.4

0

2.19

3.41

Healthy

30

B.5

0

1.66

2.96

Healthy

30

F.5

0

1.81

0.87

Healthy

29

B.6

0

2.21

2.87

Healthy

25

F.6

Low

0

1.83

0.11

Healthy

30

Vaccination -3 dpi

Vaccination -3 dpi*

C.1

Low

< assay

0

4.17

Healthy

33

G.1

High

7.79

2.75

8.33

unrelated inf.

22

C.2

Low

0

1.65

3.74

Healthy

32

G.2

Low

8.01

0

8.23

Healthy

28

C.3

0

1.24

3.45

Healthy

33

G.3

Low

2.75

4.29

5.13

Healthy

28

C.4

Low

0.5

1.45

3.43

Healthy

32

G.4

1.06

3.80

4.78

Healthy

24

C.5

Low

< assay

1.35

3.16

Healthy

32

G.5

Low

2.63

1.74

4.08

Healthy

24

C.6

0

0

2.51

Healthy

33

G.6

1.98

1.12

3.35

Healthy

24

Vaccination -1 dpi*

Vaccination -1 dpi*

D.1

High

7.28

0

8.69

Euthanized

19

H.1

High

7.51

0

8.69

Euthanized

19

D.2

Moderate

7.75

4.26

8.68

Euthanized

12

H.2

High

7.98

0

8.21

Euthanized

12

D.3

Moderate

3.03

< assay

5.62

Healthy

23

H.3

Moderate

7.99

4.16

8.16

Euthanized

11

D.4

Low

2.28

1.02

5.10

unrelated inf.

19

H.4

Low

4.65

0

5.43

Healthy

23

D.5

Low

0.76

2.53

4.15

Healthy

23

H.5

Low

2.59

1.00

4.41

Healthy

23

D.6

Low

< assay

< assay

2.77

Healthy

23

H.6

Low

2.67

1.58

4.16

Healthy

23

  1. *Naïve animals co-housed together with these groups became CSFV infected.
  2. Pigs were mock-vaccinated or vaccinated at 1, 3, or 5 days before inoculation with (A) the CBR/93 or (B) the UK2000/7.1 field strains. Blood viraemia is recorded as not detectable (), low, moderate or high. The amount of CBR/93, UK20007.1 or C-strain vaccine RNA determined using differential real-time qRT-PCR or of total CSFV RNA quantified using a routine real-time qRT-PCR assay is shown as log10 viral RNA copy per ng 18S RNA. Bold type indicates high copy number. Samples with high Ct values below the quantifiable limit of an assay (< assay) or no Ct value (0) are indicated. The clinical outcome shows those animals that reached the humane endpoint and were euthanized. Animals indicated as healthy had clinical scores that did not exceed 4. Tonsil samples were obtained on the indicated days post inoculation (dpi).