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Table 2 Effect of dietary treatment on DMI, reticular pH, daily average of time spent below the three reticular pH thresholds, ruminal volatile fatty acid (VFA) N-NH3 and lipopolysaccharides (LPS)

From: Use of dicarboxylic acids and polyphenols to attenuate reticular pH drop and acute phase response in dairy heifers fed a high grain diet

 

Treatment 1

SEM

P -value

Item

CT

FM

PM

  

DMI, 2 kg/d

14.5a

13.4b

14.7a

0.62

0.021

Reticular pH

     

  Average

6.04

6.11

6.09

0.067

0.466

  Maximum

6.61

6.54

6.55

0.063

0.569

  Nadir

5.40b

5.69a

5.62ab

0.106

0.037

  pH <5.6, 3 min

199a

16b

18b

-

0.022

  pH <5.8, 3 min

360

190

171

-

0.311

pH <6.3, min

1156

1118

1071

86.4

0.546

VFA, mM

     

  Acetate

59.2

60.9

60.6

3.60

0.911

  Propionate

26.5

30.8

27.9

2.07

0.249

  Butyrate

10.5

10.7

9.8

0.69

0.683

Ac:Pr4

2.05α

1.66β

1.77αβ

0.125

0.048

N-NH3, mg/dL

50.1

54.5

31.1

9.70

0.303

LPS5, 6, × 103 EU/mL

15.7

6.0

8.9

(5.3–16.7)

0.172

  1. 1Treatments: control diet (CT); fumarate-malate mixture (FM); polyphenol-essential oil mixture (PM).
  2. 2For DMI the repeated effect of day was significant (P <0.001).
  3. 3P-values and superscript letters (a, b: P <0.05) are given by using the non-parametric Kruskal–Wallis test and the Dunn’s multiple pairwise comparison.
  4. 4Acetate to propionate ratio.
  5. 5Statistical analysis was conducted on natural logarithm (ln) transformed data that are presented as ln back transformed and 95%-confidence interval in brackets.
  6. 6LPS were reported as endotoxin unit.
  7. a, bMeans with different superscripts within a row differ (P <0.05).
  8. α, βMeans with different superscripts within a row differ (P <0.10).