Table 2 Primer sequences for identification and detection of the virulence associated genes in P. multocida strains
Gene | Primer sequence | Accession number | Reference |
|---|---|---|---|
kmt1 | F - ATCCGCTATTTACCCAGTGG | [GenBank:NC_017027] | [21] |
R - GCTGTAAACGAACTCGCCAC | |||
hyaC-hyaD | F - TGCCAAAATCGCAGTCAG | [GenBank:NZ_CM002276] | [22] |
R - TTGCCATCATTGTCAGTG | |||
pfhA | F - AGCTGATCAAGTGGTGAAC | [GenBank:NC_016808] | This study |
R - TGGTACATTGGTGAATGCTG | |||
nanH | F - GAATATTTGGGCGGCAACA | [GenBank:NC_017764] | [23] |
R - TTCTCGCCCTGTCATCACT | |||
hgbA | F - TGGCGGATAGTCATCAAG | [GenBank:NZ_CM002276] | [23] |
R - CCAAAGAACCACTACCCA | |||
ompH | F - CGCGTATGAAGGTTTAGGT | [GenBank:NZ_CM001580] | [23] |
R - TTTAGATTGTGCGTAGTCAAC | |||
ptfA | F - TCCACTCGTTGTGGCATTCA | [GenBank:NC_017027] | This study |
R - AGAAACACCTTGAGCTGCGT |