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Table 1 Detection of viral RNA (Ct values) in EDTA blood from 3 heifers in early pregnancy on days 8 to 14 of the infection phase

From: Transmission of border disease virus from a persistently infected calf to seronegative heifers in early pregnancy

  

Ct value

DayTag

Assay

Heifer 3

Heifer 4

Heifer 5

8

Primary

Negative

37.8

40.6

Follow-up

Negative

39.7

Negative

10

Primary

40.7

Negative

39.6

Follow-up

Negative

Negative

41.7

12

Primary

Negative

Negative

42.5

Follow-up

Negative

Negative

Negative

14

Primary

Negative

Negative

40.8

Follow-up

Negative

Negative

Negative

  1. Ct values of >30 were considered weakly positive.
  2. Viral RNA was not isolated from heifers 1, 2 and 6.
  3. Viral RNA was not isolated from heifers 3, 4 and 5 on days 0 to 6 and 16 to 20.