Table 4 Identification of the selected polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) fragments
PCR-DGGE band | Nearest match (GenBank accession no.; % sequence similarity) | Taxonomic classification |
|---|---|---|
Total bacterial community analysis | Â | |
1 | Pasteurella testudinis (NR_042889; 90%) | Unclassified Pasteurellaceae |
2 | Bergeriella denitrificans (NR_040933; 99%) | Bergeriella denitrificans |
3 | Bergeriella denitrificans (NR_040933; 99%) | Bergeriella denitrificans |
4 | Clostridium lavalense (NR_044289; 93%) | Unclassified Clostridiaceae |
5 | Neisseria weaveri (NR_025902; 99%) | Neisseria weaveri |
6 | Neisseria weaveri (NR_025902; 98%) | Neisseria weaveri |
7 | Neisseria weaveri (NR_025902; 99%) | Neisseria weaveri |
8 | Neisseria weaveri (NR_025902; 98%) | Neisseria weaveri |
9 | Neisseria flavescens (KF030235; 100%) | Neisseria flavescens |
10 | Clostridium citroniae (NR_043681; 90%) | Unclassified Clostridiaceae |
11 | Ruminobacter amylophilus (NR_026450; 99%) | Ruminobacter amylophilus |
12 | Neisseria flavescens (KF030235; 100%) | Neisseria flavescens |
13 | Neisseria flavescens (KF030235; 100%) | Neisseria flavescens |
14 | Neisseria weaveri (NR_025902; 98%) | Neisseria weaveri |
15 | Howardella ureilytica (NR_044022; 94%) | Unclassified Clostridiaceae |
16 | Roseburia faecis (NR_042832; 90%) | Unclassified Lachnospiraceae |
17 | Butyrivibrio hungatei (NR_025525; 90%) | Unclassified Lachnospiraceae |
18 | Butyrivibrio hungatei (NR_025525; 93%) | Unclassified Lachnospiraceae |
19 | Ruminococcus torques (NR_036777; 90%) | Unclassified Lachnospiraceae |
Butyrivibrio-specific analysis | Â | |
20 | Butyrivibrio proteoclasticus (NR_102893; 92%) | Unclassified Lachnospiraceae |
21 | Butyrivibrio proteoclasticus (NR_102893; 98%) | Butyrivibrio proteoclasticus |
22 | Butyrivibrio proteoclasticus (NR_102893; 99%) | Butyrivibrio proteoclasticus |
23 | Robinsoniella peoriensis (NR_041882; 94%) | Unclassified Lachnospiraceae |
24 | Eubacterium ruminantium (NR_024661; 92%) | Unclassified Lachnospiraceae |