Figure 1

Pre-absorbed immunoproteomics on picsine S. agalactiae GD201008-001. (A) GD201008-001 total cell proteins (pH 4-7, 13 cm), stained with Coomassie brilliant blue G-250. (B) 2-DE blot of GD201008-001 proteins probed with untreated antiserum. (C) 2-DE blot of GD201008-001 proteins probed with “pre-absorbed” antiserum. (AB) Merge of layers A and B. In Photoshop CS, the duplicated gel layer A was used as the background layer and the western blotting layer B as the surface layer in 50% transparency. (AC) Merge of layers A and C. In Photoshop CS, the duplicated gel layer A was used as the background layer and the western blotting layer C as the surface layer in 50% transparency.