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Table 1 Peptide sequences from LC-MS/MS confirming identify of protein C

From: Purification of protein C from canine plasma

Band Sequence Probability Ion score Identity score
HCα (R)DAcEGDSGGPMVTSFR(G) 95% 62.74 25.00
  (R)DAcEGDSGGPmVTSFR(G) 95% 77.48 25.00
  (R)eLTQVGQETVVTGWGYR(S) 95% 50.45 31.31
  (R)ELTQVGQETVVTGWGYR(S) 95% 39.26 31.38
  (R)ELTQVGQETVVTGWGYR(S) 95% 47.03 31.38
  (R)ELTQVGQETVVTGWGYR(S) 95% 70.81 31.38
  (K)GEmDVDIK(E) 95% 49.08 27.51
  (R)GESPWQVVLLDSK(K) 95% 71.42 30.85
  (R)GESPWQVVLLDSKK(K) 95% 30.54 30.09
  (R)GESPWQVVLLDSKK(K) 95% 63.18 30.09
  (R)GTWFLVGLVSWGEGcGR(L) 95% 67.5 31.20
  (R)GTWFLVGLVSWGEGcGR(L) 95% 59.65 31.20
  (R)HcScAPGYR(L) 95% 30.75 25.00
  (R)LGEYDLR(R) 95% 37.33 28.93
  (R)LGEYDLR(R) 95% 37.66 28.70
  (R)LGEYDLR(R) 95% 38.07 28.93
  (R)LGEYDLR(R) 95% 41.16 28.70
  (R)LVNGKVTR(R) 95% 51.78 27.82
  (R)RGESPWQVVLLDSK(K) 95% 40.51 30.07
  (R)RGESPWQVVLLDSK(K) 95% 41.43 30.25
  (R)RGESPWQVVLLDSK(K) 95% 41.91 30.07
  (R)RGESPWQVVLLDSK(K) 95% 52.68 30.25
  (R)RGESPWQVVLLDSK(K) 95% 45.81 30.22
  (R)RGESPWQVVLLDSKK(K) 95% 54.73 28.60
  (R)RGESPWQVVLLDSKK(K) 95% 55.67 28.61
  (K)STTDNDIALLHLAQPAIFSQTIVPIcLPDSGLAER(E) 95% 58.73 26.59
  (R)YLDWIHSHIR(G) 95% 38.67 30.04
  (R)YLDWIHSHIR(G) 95% 42.28 30.12
  (R)YLDWIHSHIR(G) 95% 43.78 30.12
  (R)YLDWIHSHIR(G) 95% 33.04 30.12
HCβ (R)DAcEGDSGGPmVTSFR(G) 95% 82.07 25.00
  (R)DTNQTDQIDPR(L) 95% 43.85 27.71
  (R)DTNQTDQIDPR(L) 95% 44.08 27.76
  (R)DTNQTDQIDPR(L) 95% 43.09 26.50
  (R)ELTQVGQETVVTGWGYR(S) 95% 37.28 31.21
  (R)eLTQVGQETVVTGWGYR(S) 95% 40.06 31.36
  (R)eLTQVGQETVVTGWGYR(S) 95% 57.46 31.32
  (R)ELTQVGQETVVTGWGYR(S) 95% 45.45 31.38
  (R)ELTQVGQETVVTGWGYR(S) 95% 72.84 31.35
  (R)ELTQVGQETVVTGWGYR(S) 95% 50.99 31.29
  (R)ELTQVGQETVVTGWGYR(S) 95% 82.28 31.46
  (R)eLTQVGQETVVTGWGYR(S) 95% 82.84 31.33
  (K)EVLIHPNYSK(S) 95% 38.95 29.96
  (K)EVLIHPNYSK(S) 95% 53.99 29.85
  (K)GEmDVDIK(E) 95% 48.54 27.81
  (R)GESPWQVVLLDSK(K) 95% 83.15 30.54
  (R)GTWFLVGLVSWGEGcGR(L) 95% 62.12 31.30
  (R)GTWFLVGLVSWGEGcGR(L) 95% 52.34 31.20
  (R)LGDDHLQcQPAVK(F) 95% 29.27 30.38
  (R)LGEYDLR(R) 95% 36.72 28.93
  (R)LGEYDLR(R) 95% 38.59 28.93
  (R)RGESPWQVVLLDSK(K) 95% 43.76 30.07
  (R)RGESPWQVVLLDSK(K) 95% 48.35 30.24
  (R)RGESPWQVVLLDSK(K) 95% 52.51 30.22
  (R)RGESPWQVVLLDSK(K) 95% 45.78 30.20
  (R)RGESPWQVVLLDSKK(K) 95% 56.17 28.62
  (R)YLDWIHSHIR(G) 95% 27.58 30.04
  (R)YLDWIHSHIR(G) 95% 45.55 29.51
HCγ (R)DAcEGDSGGPmVTSFR(G) 95% 55.88 25.00
  (R)eLTQVGQETVVTGWGYR(S) 95% 74.58 31.37
  (R)ELTQVGQETVVTGWGYR(S) 95% 84.14 31.35
  (R)GEEASLENQVP(-) 95% 36.07 27.59
  (R)GESPWQVVLLDSK(K) 95% 54.73 30.81
  (R)GTWFLVGLVSWGEGcGR(L) 95% 44.16 31.21
  (R)GTWFLVGLVSWGEGcGR(L) 95% 71.82 31.35
  (R)LGEYDLR(R) 95% 39.46 28.78
  (R)RGESPWQVVLLDSK(K) 95% 48.08 30.23
  (R)RGESPWQVVLLDSK(K) 95% 44.88 30.07
  (R)YLDWIHSHIR(G) 95% 39.9 30.10
LC (R)DTnQTDQIDPR(L) 95% 48.54 26.91
  (R)ELTQVGQETVVTGWGYR(S) 95% 36.27 31.38
  (R)ELTQVGQETVVTGWGYR(S) 95% 89.79 31.45
  (R)GTWFLVGLVSWGEGcGR(L) 95% 75.93 31.30
  (R)HcScAPGYR(L) 95% 33.4 25.00
  (R)HcScAPGYR(L) 95% 42.26 25.00
  (R)HcScAPGYR(L) 95% 45.5 25.00
  (R)LGEYDLR(R) 95% 39.5 28.78
  (R)RGESPWQVVLLDSK(K) 95% 47.81 30.23
  (R)RGESPWQVVLLDSK(K) 95% 53.34 30.22
HCd (R)DAcEGDSGGPmVTSFR(G) 95% 81.66 25.00
  (R)DTnQTDQIDPR(L) 95% 42.7 26.58
  (R)ELTQVGQETVVTGWGYR(S) 95% 41.89 31.35
  (R)ELTQVGQETVVTGWGYR(S) 95% 85.33 31.30
  (R)eLTQVGQETVVTGWGYR(S) 95% 93.64 31.37
  (K)EVLIHPnYSK(S) 95% 39.21 30.08
  (K)EVLIHPnYSK(S) 95% 47.75 29.87
  (K)eVLIHPnYSK(S) 95% 57.75 28.94
  (K)EVLIHPnYSK(S) 95% 60.43 30.12
  (R)GESPWQVVLLDSK(K) 95% 67.05 30.81
  (R)GTWFLVGLVSWGEGcGR(L) 95% 69.72 31.25
  (R)HcScAPGYR(L) 95% 29.11 25.00
  (R)RGESPWQVVLLDSK(K) 95% 39.91 30.23
  (R)RGESPWQVVLLDSK(K) 95% 39.82 30.23
  (R)RGESPWQVVLLDSKK(K) 95% 54.93 28.60
  (R)YLDWIHSHIR(G) 95% 30.81 30.12
  (R)YLDWIHSHIR(G) 95% 43.69 30.11
LCd (R)ELTQVGQETVVTGWGYR(S) 95% 32.58 31.36
  (R)ELTQVGQETVVTGWGYR(S) 95% 85.2 31.30
  (R)HcScAPGYR(L) 95% 31.91 25.00
  (R)HcScAPGYR(L) 95% 32.88 25.00
  (R)LGEYDLR(R) 95% 41.34 28.93
X (R)DTNQTDQIDPR(L) 95% 33.05 27.71
  (R)ELTQVGQETVVTGWGYR(S) 95% 25.29 31.31
  (R)ELTQVGQETVVTGWGYR(S) 95% 90.91 31.38
  (R)GEEASLENQVP(-) 95% 34.55 27.73
  (R)GESPWQVVLLDSK(K) 95% 67.83 30.85
  (R)LGDDHLQcQPAVK(F) 95% 23.14 30.39
  (R)LGEYDLR(R) 95% 37.69 28.78
  (R)RGESPWQVVLLDSK(K) 95% 42.97 30.05
  (R)RGESPWQVVLLDSK(K) 95% 40.33 30.07
  (R)YLDWIHSHIR(G) 95% 39.48 29.71
Y (R)ELTQVGQETVVTGWGYR(S) 95% 31.23 31.29
  (R)ELTQVGQETVVTGWGYR(S) 95% 67.41 31.30
  (R)HcScAPGYR(L) 95% 28.9 25.00
  (R)LGDDHLQcQPAVK(F) 95% 24.31 30.32
  (R)LGEYDLR(R) 95% 41.34 28.93
  (R)RGESPWQVVLLDSK(K) 95% 25.09 30.05
Z (R)ELTQVGQETVVTGWGYR(S) 95% 31.01 31.35
  (R)ELTQVGQETVVTGWGYR(S) 95% 97.66 31.32
  (K)GEmDVDIK(E) 95% 38.24 27.82
  (R)GESPWQVVLLDSK(K) 95% 75.58 30.85
  (R)LGDDHLQcQPAVK(F) 95% 40.02 30.39
  (R)LGEYDLR(R) 95% 41.54 28.93
  (R)RGESPWQVVLLDSK(K) 95% 28.86 30.07
  (R)RGESPWQVVLLDSK(K) 95% 41.52 30.24
  (R)YLDWIHSHIR(G) 95% 25.66 30.11
  1. Bands as shown in Figure 3. Letters in lower case indicate either oxidation of methionine (m), carbamidomethyl cysteine (c), deamidation of asparagine (n) or pyroglutamate (e).